Method development and characterisation of the low-molecular-weight peptidome of human wound fluids

Mariena Ja van der Plas, Jun Cai, Jitka Petrlova, Karim Saleh, Sven Kjellström, Artur Schmidtchen

Abstract

The normal wound healing process is characterised by proteolytic events, whereas infection results in dysfunctional activations by endogenous and bacterial proteases. Peptides, downstream reporters of these proteolytic actions, could therefore serve as a promising tool for diagnosis of wounds. Using mass-spectrometry analyses, we here for the first time characterise the peptidome of human wound fluids. Sterile post-surgical wound fluids were found to contain a high degree of peptides in comparison to human plasma. Analyses of the peptidome from uninfected healing wounds and Staphylococcus aureus -infected wounds identify unique peptide patterns of various proteins, including coagulation and complement factors, proteases, and antiproteinases. Together, the work defines a workflow for analysis of peptides derived from wound fluids and demonstrates a proof-of-concept that such fluids can be used for analysis of qualitative differences of peptide patterns from larger patient cohorts, providing potential biomarkers for wound healing and infection.

OriginalsprogEngelsk
Artikelnummere66876
TidsskrifteLife
Vol/bind10
ISSN2050-084X
DOI
StatusUdgivet - 6 jul. 2021

Fingeraftryk

Dyk ned i forskningsemnerne om 'Method development and characterisation of the low-molecular-weight peptidome of human wound fluids'. Sammen danner de et unikt fingeraftryk.

Citationsformater