TY - JOUR
T1 - Method development and characterisation of the low-molecular-weight peptidome of human wound fluids
AU - van der Plas, Mariena Ja
AU - Cai, Jun
AU - Petrlova, Jitka
AU - Saleh, Karim
AU - Kjellström, Sven
AU - Schmidtchen, Artur
N1 - © 2021, van der Plas et al.
PY - 2021/7/6
Y1 - 2021/7/6
N2 - The normal wound healing process is characterised by proteolytic events, whereas infection results in dysfunctional activations by endogenous and bacterial proteases. Peptides, downstream reporters of these proteolytic actions, could therefore serve as a promising tool for diagnosis of wounds. Using mass-spectrometry analyses, we here for the first time characterise the peptidome of human wound fluids. Sterile post-surgical wound fluids were found to contain a high degree of peptides in comparison to human plasma. Analyses of the peptidome from uninfected healing wounds and Staphylococcus aureus -infected wounds identify unique peptide patterns of various proteins, including coagulation and complement factors, proteases, and antiproteinases. Together, the work defines a workflow for analysis of peptides derived from wound fluids and demonstrates a proof-of-concept that such fluids can be used for analysis of qualitative differences of peptide patterns from larger patient cohorts, providing potential biomarkers for wound healing and infection.
AB - The normal wound healing process is characterised by proteolytic events, whereas infection results in dysfunctional activations by endogenous and bacterial proteases. Peptides, downstream reporters of these proteolytic actions, could therefore serve as a promising tool for diagnosis of wounds. Using mass-spectrometry analyses, we here for the first time characterise the peptidome of human wound fluids. Sterile post-surgical wound fluids were found to contain a high degree of peptides in comparison to human plasma. Analyses of the peptidome from uninfected healing wounds and Staphylococcus aureus -infected wounds identify unique peptide patterns of various proteins, including coagulation and complement factors, proteases, and antiproteinases. Together, the work defines a workflow for analysis of peptides derived from wound fluids and demonstrates a proof-of-concept that such fluids can be used for analysis of qualitative differences of peptide patterns from larger patient cohorts, providing potential biomarkers for wound healing and infection.
KW - Body Fluids/metabolism
KW - Humans
KW - Mass Spectrometry/methods
KW - Molecular Weight
KW - Peptide Fragments/analysis
KW - Proteomics/methods
KW - Staphylococcal Infections/physiopathology
KW - Staphylococcus aureus/physiology
KW - Wound Healing/physiology
UR - http://www.scopus.com/inward/record.url?scp=85110990648&partnerID=8YFLogxK
U2 - 10.7554/eLife.66876
DO - 10.7554/eLife.66876
M3 - Journal article
C2 - 34227939
SN - 2050-084X
VL - 10
JO - eLife
JF - eLife
M1 - e66876
ER -