Metabolism of phenacetin in V79 Chinese hamster cell cultures expressing rat liver cytochrome P4501A2 compared to isolated rat hepatocytes

K G Jensen; S Loft; J Doehmer; H E Poulsen

doi:10.1016/0006-2952(93)90265-x

Metabolism of phenacetin in V79 Chinese hamster cell cultures expressing rat liver cytochrome P4501A2 compared to isolated rat hepatocytes

K G Jensen, S Loft, J Doehmer, H E Poulsen

10 Citationer (Scopus)

Abstract

In a genetically engineered V79 cell line (XEMd-MZ) expressing rat cytochrome P4501A2 the activity of phenacetin-O-deethylase was determined and compared with freshly isolated rat hepatocytes. In the V79 cells the apparent Km was 0.99 microM (N = 4), compared to the high affinity Km (0.23 microM, N = 4) found in freshly isolated rat hepatocytes, where as the Vmax found in the XEMd-MZ cells (14.90 pmol/min/10(6) cells) corresponded to the Vmax for the high affinity site of the hepatocytes (18.2 pmol/min/10(-6) cells). The metabolic capacity of phenacetin was quantitatively comparable to that of freshly isolated hepatocytes on a cell to cell basis.

Originalsprog	Engelsk
Tidsskrift	Biochemical Pharmacology
Vol/bind	45
Udgave nummer	5
Sider (fra-til)	1171-3
Antal sider	3
ISSN	0006-2952
DOI	https://doi.org/10.1016/0006-2952(93)90265-x
Status	Udgivet - 9 mar. 1993

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10.1016/0006-2952(93)90265-x

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@article{b92d6b180b3a403c97b974c915c85a67,

title = "Metabolism of phenacetin in V79 Chinese hamster cell cultures expressing rat liver cytochrome P4501A2 compared to isolated rat hepatocytes",

abstract = "In a genetically engineered V79 cell line (XEMd-MZ) expressing rat cytochrome P4501A2 the activity of phenacetin-O-deethylase was determined and compared with freshly isolated rat hepatocytes. In the V79 cells the apparent Km was 0.99 microM (N = 4), compared to the high affinity Km (0.23 microM, N = 4) found in freshly isolated rat hepatocytes, where as the Vmax found in the XEMd-MZ cells (14.90 pmol/min/10(6) cells) corresponded to the Vmax for the high affinity site of the hepatocytes (18.2 pmol/min/10(-6) cells). The metabolic capacity of phenacetin was quantitatively comparable to that of freshly isolated hepatocytes on a cell to cell basis.",

keywords = "Animals, Cells, Cultured, Cricetinae, Cricetulus, Cytochrome P-450 CYP1A2, Cytochrome P-450 Enzyme System/genetics, Liver/cytology, Male, Oxidoreductases/genetics, Phenacetin/metabolism, Rats, Rats, Wistar, Transfection",

author = "Jensen, {K G} and S Loft and J Doehmer and Poulsen, {H E}",

year = "1993",

month = mar,

day = "9",

doi = "10.1016/0006-2952(93)90265-x",

language = "English",

volume = "45",

pages = "1171--3",

journal = "Biochemical Pharmacology",

issn = "0006-2952",

publisher = "Elsevier Inc",

number = "5",

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TY - JOUR

T1 - Metabolism of phenacetin in V79 Chinese hamster cell cultures expressing rat liver cytochrome P4501A2 compared to isolated rat hepatocytes

AU - Jensen, K G

AU - Loft, S

AU - Doehmer, J

AU - Poulsen, H E

PY - 1993/3/9

Y1 - 1993/3/9

N2 - In a genetically engineered V79 cell line (XEMd-MZ) expressing rat cytochrome P4501A2 the activity of phenacetin-O-deethylase was determined and compared with freshly isolated rat hepatocytes. In the V79 cells the apparent Km was 0.99 microM (N = 4), compared to the high affinity Km (0.23 microM, N = 4) found in freshly isolated rat hepatocytes, where as the Vmax found in the XEMd-MZ cells (14.90 pmol/min/10(6) cells) corresponded to the Vmax for the high affinity site of the hepatocytes (18.2 pmol/min/10(-6) cells). The metabolic capacity of phenacetin was quantitatively comparable to that of freshly isolated hepatocytes on a cell to cell basis.

AB - In a genetically engineered V79 cell line (XEMd-MZ) expressing rat cytochrome P4501A2 the activity of phenacetin-O-deethylase was determined and compared with freshly isolated rat hepatocytes. In the V79 cells the apparent Km was 0.99 microM (N = 4), compared to the high affinity Km (0.23 microM, N = 4) found in freshly isolated rat hepatocytes, where as the Vmax found in the XEMd-MZ cells (14.90 pmol/min/10(6) cells) corresponded to the Vmax for the high affinity site of the hepatocytes (18.2 pmol/min/10(-6) cells). The metabolic capacity of phenacetin was quantitatively comparable to that of freshly isolated hepatocytes on a cell to cell basis.

KW - Animals

KW - Cells, Cultured

KW - Cricetinae

KW - Cricetulus

KW - Cytochrome P-450 CYP1A2

KW - Cytochrome P-450 Enzyme System/genetics

KW - Liver/cytology

KW - Male

KW - Oxidoreductases/genetics

KW - Phenacetin/metabolism

KW - Rats

KW - Rats, Wistar

KW - Transfection

U2 - 10.1016/0006-2952(93)90265-x

DO - 10.1016/0006-2952(93)90265-x

M3 - Journal article

C2 - 8461047

SN - 0006-2952

VL - 45

SP - 1171

EP - 1173

JO - Biochemical Pharmacology

JF - Biochemical Pharmacology

IS - 5

ER -

Metabolism of phenacetin in V79 Chinese hamster cell cultures expressing rat liver cytochrome P4501A2 compared to isolated rat hepatocytes

Abstract

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