Metabolic Imaging of Breast Cancer and the Normal Brain: Application of Hyperpolarized 13C Magnetic Resonance Spectroscopy

Sadia Asghar Butt


Cellular metabolism is a set of biochemical reactions that happen in living organisms to maintain life. Enzymes act as catalysts and allow these reactions to proceed quickly and efficiently in order to maintain the cellular function and reproduction.
Metabolic Magnetic Resonance Spectroscopy (MRS) of hyperpolarized substrates enables the visualization, characterization, and quantification of biological processes taking without perturbing them. Biologic processes can, thus, be studied in their own physiologically authentic environment. This ability to measure fine metabolic changes opens up an incredible number of exciting possibilities for medical application, including early detection of disease. Such early detection allows for personalized treatment, which may increase the chances for a successful outcome.
This PhD thesis is based on experimental studies on the cellular metabolism using MRS in two biological systems - breast cancer and normal brain. Breast cancer metabolism was longitudinally monitored in a mouse model using MRS of hyperpolirized pyruvate. The results demonstrated that we could monitor the changes in metabolism with increasing disease severity. The normal cerebral metabolism of α-ketoisocaproate (KIC) was studied in the rat brain. The findings showed that hyperpolarized KIC is a promising substrate for in vivo evaluation of specific enzymatic activity.

ForlagFaculty of Health and Medical Sciences, University of Copenhagen
Antal sider84
StatusUdgivet - 30 maj 2012