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Udgivet

Memory T helper cells identify patients with nickel, cobalt, and chromium metal allergy

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

DOI

  1. Incidence rates of occupational contact dermatitis in Denmark between 2007 and 2018 - A population-based study

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Aluminium contact allergy without vaccination granulomas: A systematic review and meta-analysis

    Publikation: Bidrag til tidsskriftReviewForskningpeer review

  3. Nickel release from metallic earrings: A survey of the Danish market and validation of the nickel spot test

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Decrease of contact allergy to hydroxyisohexyl 3-cyclohexene carboxaldehyde in Europe prior to its ban and diagnostic value

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Incidence rates of occupational contact dermatitis in Denmark between 2007 and 2018 - A population-based study

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Increased all-cause mortality in concomitant atopic dermatitis and asthma: A nationwide registry-based study

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Aluminium contact allergy without vaccination granulomas: A systematic review and meta-analysis

    Publikation: Bidrag til tidsskriftReviewForskningpeer review

  4. Chemicals in moisturizers may promote type 2 inflammation and food allergy

    Publikation: Bidrag til tidsskriftLetterForskningpeer review

Vis graf over relationer

Background: Patch testing is the gold standard for identifying culprit allergens in allergic contact dermatitis; however, it is laborious and positive reactions are difficult to quantitate. Development of complementary in vitro tests is, therefore, of great importance. Objectives: This study aimed to improve the in vitro lymphocyte proliferation test (LPT) to detect allergic responses to nickel (Ni), cobalt (Co), and chromium (Cr). Methods: Twenty-one metal allergic patients with a positive patch test to Ni (n=16), Co (n=8), and Cr (n=3) and 13 controls were included. All were tested by a flow cytometric LPT. Results: Metal-reactive cells were identified as T helper (Th) cells with high expression of the memory marker CD45RO. Skin-homing (cutaneous lymphocyte-associated antigen positive [CLA+]) Ni-reactive memory Th (Thmemhi) cells identified individuals with a positive patch test for Ni with 100% sensitivity (95% confidence interval [CI] 81%-100%) and 92% specificity (95% CI 67%-100%). Moreover, Co-specific Thmemhi cells expressing CCR6 identified patients with a positive patch test for Co with 63% sensitivity (95% CI 31%-86%) and 100% specificity (95% CI 77%-100%). In Cr allergic individuals, Cr-reactive Thmemhi cells tended to increased CLA and CCR6 expression. Conclusion: Metal-reactive Th cells with high expression of CD45RO and coexpression of CLA and CCR6 improved the LPT, making it an attractive supplement to the patch test.

OriginalsprogEngelsk
TidsskriftContact Dermatitis
Vol/bind85
Udgave nummer1
Sider (fra-til)7-16
Antal sider10
ISSN0105-1873
DOI
StatusUdgivet - jul. 2021

Bibliografisk note

Publisher Copyright:
© 2021 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd

Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.

ID: 66081414