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Region Hovedstaden - en del af Københavns Universitetshospital
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Measuring histamine and cytokine release from basophils and mast cells

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Vis graf over relationer

Basophils and mast cells are known for their capability to release both preformed and newly synthesized inflammatory mediators. In this chapter we describe how to stimulate and detect histamine released from basophils in whole blood, purified basophils, in vitro cultured mast cells, and in situ skin mast cells. We also give an example of an activation protocol for basophil and mast cell cytokine release and discuss approaches for cytokine detection.

OriginalsprogEngelsk
TidsskriftMethods in molecular biology
Vol/bind1192
Sider (fra-til)135-45
Antal sider11
ISSN1064-3745
DOI
StatusUdgivet - 2014

ID: 44519776