Measuring and evaluating interferon beta-induced antibodies in patients with multiple sclerosis

C Ross; K M Clemmesen; P S Sørensen; Nils Koch-Henriksen; K Bendtzen

Measuring and evaluating interferon beta-induced antibodies in patients with multiple sclerosis

C Ross, K M Clemmesen, P S Sørensen, Nils Koch-Henriksen, K Bendtzen

34 Citationer (Scopus)

Abstract

Administration of interferons (IFNs) may induce antibodies that interfere with therapeutic efficacy. We have optimized and validated methods for large-scale economic screening. Sera from patients with relapsing-remitting multiple sclerosis (MS) were investigated for binding antibody (BAb) by protein-G affinity-chromatography radioimmunoassay and a commercially available enzyme immunoassay (EIA). Neutralizing antibody (NAb) was investigated by cytopathic effect assays (CEA) using both fixed amount and serially diluted sera. BAb correlated with log10-transformed titres obtained by EIA (r=0.70, P

Originalsprog	Engelsk
Tidsskrift	Multiple Sclerosis
Vol/bind	12
Udgave nummer	1
Sider (fra-til)	39-46
Antal sider	8
ISSN	1352-4585
Status	Udgivet - 1 feb. 2006

Citationsformater

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AU - Ross, C

AU - Clemmesen, K M

AU - Sørensen, P S

AU - Koch-Henriksen, Nils

AU - Bendtzen, K

PY - 2006/2/1

Y1 - 2006/2/1

N2 - Administration of interferons (IFNs) may induce antibodies that interfere with therapeutic efficacy. We have optimized and validated methods for large-scale economic screening. Sera from patients with relapsing-remitting multiple sclerosis (MS) were investigated for binding antibody (BAb) by protein-G affinity-chromatography radioimmunoassay and a commercially available enzyme immunoassay (EIA). Neutralizing antibody (NAb) was investigated by cytopathic effect assays (CEA) using both fixed amount and serially diluted sera. BAb correlated with log10-transformed titres obtained by EIA (r=0.70, P

AB - Administration of interferons (IFNs) may induce antibodies that interfere with therapeutic efficacy. We have optimized and validated methods for large-scale economic screening. Sera from patients with relapsing-remitting multiple sclerosis (MS) were investigated for binding antibody (BAb) by protein-G affinity-chromatography radioimmunoassay and a commercially available enzyme immunoassay (EIA). Neutralizing antibody (NAb) was investigated by cytopathic effect assays (CEA) using both fixed amount and serially diluted sera. BAb correlated with log10-transformed titres obtained by EIA (r=0.70, P

M3 - Journal article

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JO - Multiple Sclerosis

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ER -

Measuring and evaluating interferon beta-induced antibodies in patients with multiple sclerosis

Abstract

Fingeraftryk

Citationsformater