Abstract
Using fragment 5-17 of human gastrin-17 extended with glycine at the C-terminus as hapten, three of six rabbits produced high-titer, high-avidity antisera specific for glycine-extended gastrins. In combination with trypsin and carboxypeptidase B cleavage, radioimmunoassays based on these antisera measured progastrins in some extra-antral tissues and certain malignant tumors. The results show that sequential cleavage with trypsin and carboxypeptidase B followed by radioimmunoassay of glycine-extended peptides is a rapid and accurate procedure for measurement of biosynthetic precursors of alpha-amidated peptide hormones. Moreover, the procedure seems promising in the search for tumor markers.
Originalsprog | Engelsk |
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Tidsskrift | Analytical Biochemistry |
Vol/bind | 152 |
Udgave nummer | 1 |
Sider (fra-til) | 119-26 |
Antal sider | 8 |
ISSN | 0003-2697 |
DOI | |
Status | Udgivet - jan. 1986 |
Udgivet eksternt | Ja |