Matrix-assisted laser desorption ionization-time of flight mass spectrometry analysis of Gram-positive, catalase-negative cocci not belonging to the Streptococcus or Enterococcus genus and benefits of database extension

Jens Jørgen Christensen, Rimtas Dargis, Monja Hammer, Ulrik Stenz Justesen, Xiaohui C Nielsen, Michael Kemp, Danish MALDI-TOF MS Study Group, Michael Tvede

59 Citationer (Scopus)

Abstract

Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry with a Bruker Daltonics microflex LT system was applied to 90 well-characterized catalase-negative, Gram-positive cocci not belonging to the streptococci or enterococci. Biotyper version 2.0.43.1 software was used singly or in combination with a database extension generated in this study with 51 collection strains from 16 genera. Most strains were identified by using both databases individually, and some were identified only by applying the combined database. Thus, the methodology is very useful and the generated database extension was helpful.
OriginalsprogEngelsk
TidsskriftJournal of Clinical Microbiology
Vol/bind50
Udgave nummer5
Sider (fra-til)1787-91
Antal sider5
ISSN0095-1137
DOI
StatusUdgivet - 2012

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