TY - JOUR
T1 - Low Sensitivity of BinaxNOW RSV in Infants
AU - Zuurbier, Roy P
AU - Bont, Louis J
AU - Langedijk, Annefleur C
AU - Hamer, Mirjam
AU - Korsten, Koos
AU - Drysdale, Simon B
AU - Snape, Matthew D
AU - Robinson, Hannah
AU - Pollard, Andrew J
AU - Martinón-Torres, Federico
AU - Rodríguez-Tenreiro Sánchez, Carmen
AU - Gómez-Carballa, Alberto
AU - Dacosta-Urbieta, Ana Isabel
AU - Heikkinen, Terho
AU - Cunningham, Steve
AU - van Houten, Marlies A
AU - Wildenbeest, Joanne G
AU - RESCEU Investigators
A2 - Fischer, Thea Kølsen
N1 - © The Author(s) 2020. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: [email protected].
PY - 2020/10/7
Y1 - 2020/10/7
N2 - BACKGROUND: Respiratory syncytial virus (RSV) is a major cause of hospitalization in infants. Early detection of RSV can optimize clinical management and minimize use of antibiotics. BinaxNOW RSV (BN) is a rapid antigen detection test that is widely used. We aimed to validate the sensitivity of BN in hospitalized and nonhospitalized infants against the gold standard of molecular diagnosis.METHODS: We evaluated the performance of BN in infants with acute respiratory tract infections with different degrees of disease severity. Diagnostic accuracy of BN test results were compared with molecular diagnosis as reference standard.RESULTS: One hundred sixty-two respiratory samples from 148 children from October 2017 to February 2019 were studied. Sixty-six (40.7%) samples tested positive for RSV (30 hospitalizations, 31 medically attended episodes not requiring hospitalization, and 5 nonmedically attended episodes). Five of these samples tested positive with BN, leading to an overall sensitivity of BN of 7.6% (95% confidence interval [CI], 3.3%-16.5%) and a specificity of 100% (95% CI, 96.2%-100%). Sensitivity was low in all subgroups.CONCLUSIONS: We found a low sensitivity of BN for point-of-care detection of RSV infection. BinaxNOW RSV should be used and interpreted with caution.
AB - BACKGROUND: Respiratory syncytial virus (RSV) is a major cause of hospitalization in infants. Early detection of RSV can optimize clinical management and minimize use of antibiotics. BinaxNOW RSV (BN) is a rapid antigen detection test that is widely used. We aimed to validate the sensitivity of BN in hospitalized and nonhospitalized infants against the gold standard of molecular diagnosis.METHODS: We evaluated the performance of BN in infants with acute respiratory tract infections with different degrees of disease severity. Diagnostic accuracy of BN test results were compared with molecular diagnosis as reference standard.RESULTS: One hundred sixty-two respiratory samples from 148 children from October 2017 to February 2019 were studied. Sixty-six (40.7%) samples tested positive for RSV (30 hospitalizations, 31 medically attended episodes not requiring hospitalization, and 5 nonmedically attended episodes). Five of these samples tested positive with BN, leading to an overall sensitivity of BN of 7.6% (95% confidence interval [CI], 3.3%-16.5%) and a specificity of 100% (95% CI, 96.2%-100%). Sensitivity was low in all subgroups.CONCLUSIONS: We found a low sensitivity of BN for point-of-care detection of RSV infection. BinaxNOW RSV should be used and interpreted with caution.
KW - Adolescent
KW - Antigens, Viral/blood
KW - Case-Control Studies
KW - Child
KW - Child, Preschool
KW - Cohort Studies
KW - Female
KW - Hospitalization/statistics & numerical data
KW - Humans
KW - Male
KW - Pathology, Molecular/methods
KW - Point-of-Care Systems
KW - Reagent Kits, Diagnostic
KW - Respiratory Syncytial Virus Infections/diagnosis
KW - Respiratory Syncytial Virus, Human/isolation & purification
KW - Sensitivity and Specificity
U2 - 10.1093/infdis/jiaa050
DO - 10.1093/infdis/jiaa050
M3 - Journal article
C2 - 32227106
SN - 0022-1899
VL - 222
SP - S640-S647
JO - The Journal of infectious diseases
JF - The Journal of infectious diseases
IS - Suppl 7
ER -