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Loss of social behaviours in populations of Pseudomonas aeruginosa infecting lungs of patients with cystic fibrosis

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Harvard

Jiricny, N, Molin, S, Foster, K, Diggle, SP, Scanlan, PD, Ghoul, M, Johansen, HK, Santorelli, LA, Popat, R, West, SA & Griffin, AS 2014, 'Loss of social behaviours in populations of Pseudomonas aeruginosa infecting lungs of patients with cystic fibrosis' P L o S One, bind 9, nr. 1, s. e83124. https://doi.org/10.1371/journal.pone.0083124

APA

Jiricny, N., Molin, S., Foster, K., Diggle, S. P., Scanlan, P. D., Ghoul, M., ... Griffin, A. S. (2014). Loss of social behaviours in populations of Pseudomonas aeruginosa infecting lungs of patients with cystic fibrosis. P L o S One, 9(1), e83124. https://doi.org/10.1371/journal.pone.0083124

CBE

Jiricny N, Molin S, Foster K, Diggle SP, Scanlan PD, Ghoul M, Johansen HK, Santorelli LA, Popat R, West SA, Griffin AS. 2014. Loss of social behaviours in populations of Pseudomonas aeruginosa infecting lungs of patients with cystic fibrosis. P L o S One. 9(1):e83124. https://doi.org/10.1371/journal.pone.0083124

MLA

Vancouver

Author

Jiricny, Natalie ; Molin, Søren ; Foster, Kevin ; Diggle, Stephen P ; Scanlan, Pauline D ; Ghoul, Melanie ; Johansen, Helle Krogh ; Santorelli, Lorenzo A ; Popat, Roman ; West, Stuart A ; Griffin, Ashleigh S. / Loss of social behaviours in populations of Pseudomonas aeruginosa infecting lungs of patients with cystic fibrosis. I: P L o S One. 2014 ; Bind 9, Nr. 1. s. e83124.

Bibtex

@article{51a28761fa934172b630ae645a1dc0ee,
title = "Loss of social behaviours in populations of Pseudomonas aeruginosa infecting lungs of patients with cystic fibrosis",
abstract = "Pseudomonas aeruginosa, is an opportunistic, bacterial pathogen causing persistent and frequently fatal infections of the lung in patients with cystic fibrosis. Isolates from chronic infections differ from laboratory and environmental strains in a range of traits and this is widely interpreted as the result of adaptation to the lung environment. Typically, chronic strains carry mutations in global regulation factors that could effect reduced expression of social traits, raising the possibility that competitive dynamics between cooperative and selfish, cheating strains could also drive changes in P. aeruginosa infections. We compared the expression of cooperative traits - biofilm formation, secretion of exo-products and quorum sensing (QS) - in P. aeruginosa isolates that were estimated to have spent different lengths of time in the lung based on clinical information. All three exo-products involved in nutrient acquisition were produced in significantly smaller quantities with increased duration of infection, and patterns across four QS signal molecules were consistent with accumulation over time of mutations in lasR, which are known to disrupt the ability of cells to respond to QS signal. Pyocyanin production, and the proportion of cells in biofilm relative to motile, free-living cells in liquid culture, did not change. Overall, our results confirm that the loss of social behaviour is a consistent trend with time spent in the lung and suggest that social dynamics are potentially relevant to understanding the behaviour of P. aeruginosa in lung infections.",
keywords = "Biofilms, Cell Communication, Cystic Fibrosis, Extracellular Space, Humans, Lung, Oligopeptides, Pancreatic Elastase, Peptide Hydrolases, Pseudomonas aeruginosa, Siderophores",
author = "Natalie Jiricny and S{\o}ren Molin and Kevin Foster and Diggle, {Stephen P} and Scanlan, {Pauline D} and Melanie Ghoul and Johansen, {Helle Krogh} and Santorelli, {Lorenzo A} and Roman Popat and West, {Stuart A} and Griffin, {Ashleigh S}",
year = "2014",
doi = "10.1371/journal.pone.0083124",
language = "English",
volume = "9",
pages = "e83124",
journal = "P L o S One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "1",

}

RIS

TY - JOUR

T1 - Loss of social behaviours in populations of Pseudomonas aeruginosa infecting lungs of patients with cystic fibrosis

AU - Jiricny, Natalie

AU - Molin, Søren

AU - Foster, Kevin

AU - Diggle, Stephen P

AU - Scanlan, Pauline D

AU - Ghoul, Melanie

AU - Johansen, Helle Krogh

AU - Santorelli, Lorenzo A

AU - Popat, Roman

AU - West, Stuart A

AU - Griffin, Ashleigh S

PY - 2014

Y1 - 2014

N2 - Pseudomonas aeruginosa, is an opportunistic, bacterial pathogen causing persistent and frequently fatal infections of the lung in patients with cystic fibrosis. Isolates from chronic infections differ from laboratory and environmental strains in a range of traits and this is widely interpreted as the result of adaptation to the lung environment. Typically, chronic strains carry mutations in global regulation factors that could effect reduced expression of social traits, raising the possibility that competitive dynamics between cooperative and selfish, cheating strains could also drive changes in P. aeruginosa infections. We compared the expression of cooperative traits - biofilm formation, secretion of exo-products and quorum sensing (QS) - in P. aeruginosa isolates that were estimated to have spent different lengths of time in the lung based on clinical information. All three exo-products involved in nutrient acquisition were produced in significantly smaller quantities with increased duration of infection, and patterns across four QS signal molecules were consistent with accumulation over time of mutations in lasR, which are known to disrupt the ability of cells to respond to QS signal. Pyocyanin production, and the proportion of cells in biofilm relative to motile, free-living cells in liquid culture, did not change. Overall, our results confirm that the loss of social behaviour is a consistent trend with time spent in the lung and suggest that social dynamics are potentially relevant to understanding the behaviour of P. aeruginosa in lung infections.

AB - Pseudomonas aeruginosa, is an opportunistic, bacterial pathogen causing persistent and frequently fatal infections of the lung in patients with cystic fibrosis. Isolates from chronic infections differ from laboratory and environmental strains in a range of traits and this is widely interpreted as the result of adaptation to the lung environment. Typically, chronic strains carry mutations in global regulation factors that could effect reduced expression of social traits, raising the possibility that competitive dynamics between cooperative and selfish, cheating strains could also drive changes in P. aeruginosa infections. We compared the expression of cooperative traits - biofilm formation, secretion of exo-products and quorum sensing (QS) - in P. aeruginosa isolates that were estimated to have spent different lengths of time in the lung based on clinical information. All three exo-products involved in nutrient acquisition were produced in significantly smaller quantities with increased duration of infection, and patterns across four QS signal molecules were consistent with accumulation over time of mutations in lasR, which are known to disrupt the ability of cells to respond to QS signal. Pyocyanin production, and the proportion of cells in biofilm relative to motile, free-living cells in liquid culture, did not change. Overall, our results confirm that the loss of social behaviour is a consistent trend with time spent in the lung and suggest that social dynamics are potentially relevant to understanding the behaviour of P. aeruginosa in lung infections.

KW - Biofilms

KW - Cell Communication

KW - Cystic Fibrosis

KW - Extracellular Space

KW - Humans

KW - Lung

KW - Oligopeptides

KW - Pancreatic Elastase

KW - Peptide Hydrolases

KW - Pseudomonas aeruginosa

KW - Siderophores

U2 - 10.1371/journal.pone.0083124

DO - 10.1371/journal.pone.0083124

M3 - Journal article

VL - 9

SP - e83124

JO - P L o S One

JF - P L o S One

SN - 1932-6203

IS - 1

ER -

ID: 45102910