Longitudinal and cross-sectional studies of HIV-1 RNA and DNA loads in blood and the female genital tract

Astrid K N Iversen; Jørn Attermann; Jan Gerstoft; Lars Fugger; James I Mullins; Peter Skinhøj

doi:10.1016/j.ejogrb.2004.05.016

Longitudinal and cross-sectional studies of HIV-1 RNA and DNA loads in blood and the female genital tract

Astrid K N Iversen, Jørn Attermann, Jan Gerstoft, Lars Fugger, James I Mullins, Peter Skinhøj

18 Citationer (Scopus)

Abstract

OBJECTIVE: To examine if correlates of HIV-1 genital shedding in cross-sectional studies can be used to determine the risk of shedding in individual HIV-1-positive women.

STUDY DESIGN: Longitudinal samples from blood and cervix were obtained from 18 HIV-1 infected women, and HIV-1 RNA and cell-associated DNA virus, and beta-chemokine levels, were measured. Associations between variables were analyzed at both individual and group level.

RESULTS: The variation over time was 2.9-, 2.1-, and 2.3-fold in plasma RNA, PBMC DNA and cervical RNA load, respectively, and reached 6.2-fold in cervical DNA load. Differences were observed between associations in individual- and group-level comparisons, suggesting that a separate reservoir of HIV replication may exist in the genital tract of some women, which is influenced by local environmental factors.

CONCLUSIONS: Our study underscores the importance of caution during contact with genital fluids at all stages of infection and disease regardless of treatment and HIV-1 blood loads.

Originalsprog	Engelsk
Tidsskrift	European journal of obstetrics, gynecology, and reproductive biology
Vol/bind	117
Udgave nummer	2
Sider (fra-til)	227-35
Antal sider	9
ISSN	0301-2115
DOI	https://doi.org/10.1016/j.ejogrb.2004.05.016
Status	Udgivet - 1 dec. 2004
Udgivet eksternt	Ja

Adgang til dokumentet

10.1016/j.ejogrb.2004.05.016

Citationsformater

@article{fd5cbb9a1da0496692da164008a30a23,

title = "Longitudinal and cross-sectional studies of HIV-1 RNA and DNA loads in blood and the female genital tract",

abstract = "OBJECTIVE: To examine if correlates of HIV-1 genital shedding in cross-sectional studies can be used to determine the risk of shedding in individual HIV-1-positive women.STUDY DESIGN: Longitudinal samples from blood and cervix were obtained from 18 HIV-1 infected women, and HIV-1 RNA and cell-associated DNA virus, and beta-chemokine levels, were measured. Associations between variables were analyzed at both individual and group level.RESULTS: The variation over time was 2.9-, 2.1-, and 2.3-fold in plasma RNA, PBMC DNA and cervical RNA load, respectively, and reached 6.2-fold in cervical DNA load. Differences were observed between associations in individual- and group-level comparisons, suggesting that a separate reservoir of HIV replication may exist in the genital tract of some women, which is influenced by local environmental factors.CONCLUSIONS: Our study underscores the importance of caution during contact with genital fluids at all stages of infection and disease regardless of treatment and HIV-1 blood loads.",

keywords = "Adolescent, Adult, Aged, Chemokines, CC/analysis, Child, Child, Preschool, Cross-Sectional Studies, DNA, Viral/analysis, Denmark, Female, Genitalia, Female/virology, HIV-1/isolation & purification, Humans, Infant, Infant, Newborn, Longitudinal Studies, Middle Aged, RNA, Viral/analysis, Viral Load/methods",

author = "Iversen, {Astrid K N} and J{\o}rn Attermann and Jan Gerstoft and Lars Fugger and Mullins, {James I} and Peter Skinh{\o}j",

year = "2004",

month = dec,

day = "1",

doi = "10.1016/j.ejogrb.2004.05.016",

language = "English",

volume = "117",

pages = "227--35",

journal = "European journal of obstetrics, gynecology, and reproductive biology",

issn = "0301-2115",

publisher = "Elsevier Ireland Ltd",

number = "2",

}

TY - JOUR

T1 - Longitudinal and cross-sectional studies of HIV-1 RNA and DNA loads in blood and the female genital tract

AU - Iversen, Astrid K N

AU - Attermann, Jørn

AU - Gerstoft, Jan

AU - Fugger, Lars

AU - Mullins, James I

AU - Skinhøj, Peter

PY - 2004/12/1

Y1 - 2004/12/1

N2 - OBJECTIVE: To examine if correlates of HIV-1 genital shedding in cross-sectional studies can be used to determine the risk of shedding in individual HIV-1-positive women.STUDY DESIGN: Longitudinal samples from blood and cervix were obtained from 18 HIV-1 infected women, and HIV-1 RNA and cell-associated DNA virus, and beta-chemokine levels, were measured. Associations between variables were analyzed at both individual and group level.RESULTS: The variation over time was 2.9-, 2.1-, and 2.3-fold in plasma RNA, PBMC DNA and cervical RNA load, respectively, and reached 6.2-fold in cervical DNA load. Differences were observed between associations in individual- and group-level comparisons, suggesting that a separate reservoir of HIV replication may exist in the genital tract of some women, which is influenced by local environmental factors.CONCLUSIONS: Our study underscores the importance of caution during contact with genital fluids at all stages of infection and disease regardless of treatment and HIV-1 blood loads.

AB - OBJECTIVE: To examine if correlates of HIV-1 genital shedding in cross-sectional studies can be used to determine the risk of shedding in individual HIV-1-positive women.STUDY DESIGN: Longitudinal samples from blood and cervix were obtained from 18 HIV-1 infected women, and HIV-1 RNA and cell-associated DNA virus, and beta-chemokine levels, were measured. Associations between variables were analyzed at both individual and group level.RESULTS: The variation over time was 2.9-, 2.1-, and 2.3-fold in plasma RNA, PBMC DNA and cervical RNA load, respectively, and reached 6.2-fold in cervical DNA load. Differences were observed between associations in individual- and group-level comparisons, suggesting that a separate reservoir of HIV replication may exist in the genital tract of some women, which is influenced by local environmental factors.CONCLUSIONS: Our study underscores the importance of caution during contact with genital fluids at all stages of infection and disease regardless of treatment and HIV-1 blood loads.

KW - Adolescent

KW - Adult

KW - Aged

KW - Chemokines, CC/analysis

KW - Child

KW - Child, Preschool

KW - Cross-Sectional Studies

KW - DNA, Viral/analysis

KW - Denmark

KW - Female

KW - Genitalia, Female/virology

KW - HIV-1/isolation & purification

KW - Humans

KW - Infant

KW - Infant, Newborn

KW - Longitudinal Studies

KW - Middle Aged

KW - RNA, Viral/analysis

KW - Viral Load/methods

U2 - 10.1016/j.ejogrb.2004.05.016

DO - 10.1016/j.ejogrb.2004.05.016

M3 - Journal article

C2 - 15541862

SN - 0301-2115

VL - 117

SP - 227

EP - 235

JO - European journal of obstetrics, gynecology, and reproductive biology

JF - European journal of obstetrics, gynecology, and reproductive biology

IS - 2

ER -

Longitudinal and cross-sectional studies of HIV-1 RNA and DNA loads in blood and the female genital tract

Abstract

Adgang til dokumentet

Fingeraftryk

Citationsformater