TY - JOUR
T1 - Long-term carriage and evolution of VREfmLong-term carriage and evolution of vancomycin-resistant Enterococcus faecium
T2 - a genomic study on consecutive isolates
AU - Knudsen, Maja Johanne Søndergaard
AU - Samaniego Castruita, Jose Alfredo
AU - Mollerup, Sarah
AU - Holzknecht, Barbara Juliane
AU - Hoppe, Morten
AU - Westh, Henrik
AU - Pinholt, Mette
AU - Rubin, Ingrid Maria Cecilia
N1 - © The Author(s) 2023. Published by Oxford University Press on behalf of British Society for Antimicrobial Chemotherapy.
PY - 2024/2
Y1 - 2024/2
N2 - OBJECTIVES: To determine if vancomycin-resistant Enterococcus faecium (VREfm) carriers carry the same VREfm clone after a minimum follow-up of 365 days. For those carrying the same clone, we investigated the genomic evolution per year per genome.METHODS: We used WGS results to assign VREfm clones to each isolate and determine clone shifts. Finally, we calculated distance in core-genome MLST alleles, and the number of SNPs between consecutive VREfm isolates from patients carrying the same VREfm clone.RESULTS: In total, 44.2% of patients carried the same VREfm clone, and the genomic evolution was 1.8 alleles and 2.6 SNPs per genome per year.CONCLUSIONS: In our population of long-term carriers, we calculated a molecular clock of 2.6 SNPs.
AB - OBJECTIVES: To determine if vancomycin-resistant Enterococcus faecium (VREfm) carriers carry the same VREfm clone after a minimum follow-up of 365 days. For those carrying the same clone, we investigated the genomic evolution per year per genome.METHODS: We used WGS results to assign VREfm clones to each isolate and determine clone shifts. Finally, we calculated distance in core-genome MLST alleles, and the number of SNPs between consecutive VREfm isolates from patients carrying the same VREfm clone.RESULTS: In total, 44.2% of patients carried the same VREfm clone, and the genomic evolution was 1.8 alleles and 2.6 SNPs per genome per year.CONCLUSIONS: In our population of long-term carriers, we calculated a molecular clock of 2.6 SNPs.
UR - http://www.scopus.com/inward/record.url?scp=85183077228&partnerID=8YFLogxK
U2 - 10.1093/jacamr/dlad153
DO - 10.1093/jacamr/dlad153
M3 - Journal article
C2 - 38161962
SN - 2632-1823
VL - 6
SP - dlad153
JO - JAC-antimicrobial resistance
JF - JAC-antimicrobial resistance
IS - 1
M1 - dlad153
ER -