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Localization of CGRP receptor components, CGRP, and receptor binding sites in human and rhesus cerebellar cortex

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Eftekhari, Sajedeh ; Salvatore, Christopher A ; Gaspar, Renee C ; Roberts, Rhonda ; O'Malley, Stacey ; Zeng, Zhizhen ; Edvinsson, Lars. / Localization of CGRP receptor components, CGRP, and receptor binding sites in human and rhesus cerebellar cortex. I: Cerebellum. 2013 ; Bind 12, Nr. 6. s. 937-49.

Bibtex

@article{43f5e78179e1441388eb1a2611ad0aae,
title = "Localization of CGRP receptor components, CGRP, and receptor binding sites in human and rhesus cerebellar cortex",
abstract = "The cerebellum is classically considered to be mainly involved in motor processing, but studies have suggested several other functions, including pain processing. Calcitonin-gene-related peptide (CGRP) is a neuropeptide involved in migraine pathology, where there is elevated release of CGRP during migraine attacks and CGRP receptor antagonists have antimigraine efficacy. In the present study, we examined CGRP and CGRP receptor binding sites and protein expression in primate cerebellar cortex. Additionally, mRNA expression of the CGRP receptor components, calcitonin receptor-like receptor (CLR) and receptor activity modifying protein 1 (RAMP1), was examined. In addition, expression of procalcitonin was studied. We observed high [(3)H]MK-3207 (CGRP receptor antagonist) binding densities in the molecular layer of rhesus cerebellar cortex; however, due to the limit of resolution of the autoradiographic image the exact cellular localization could not be determined. Similarly, [(125)I]CGRP binding was observed in the molecular layer and Purkinje cell layer of human cerebellum. CLR and RAMP1 mRNA was expressed within the Purkinje cell layer and some expression was found in the molecular layer. Immunofluorescence revealed expression of CGRP, CLR, and RAMP1 in the Purkinje cells and in cells in the molecular layer. Procalcitonin was found in the same localization. Recent research in the biology of cerebellum indicates that it may have a role in nociception. For the first time we have identified CGRP and CGRP receptor binding sites together with CGRP receptor expression through protein and mRNA localization in primate cerebellar cortex. These results point toward a functional role of CGRP in cerebellum. Further efforts are needed to evaluate this.",
keywords = "Aged, Aged, 80 and over, Animals, Binding Sites, Calcitonin Gene-Related Peptide, Cerebellar Cortex, Female, Glutamate Decarboxylase, Humans, Macaca mulatta, Male, Nerve Tissue Proteins, Postmortem Changes, Protein Binding, Radioligand Assay, Receptors, Calcitonin Gene-Related Peptide",
author = "Sajedeh Eftekhari and Salvatore, {Christopher A} and Gaspar, {Renee C} and Rhonda Roberts and Stacey O'Malley and Zhizhen Zeng and Lars Edvinsson",
year = "2013",
month = dec,
doi = "10.1007/s12311-013-0509-4",
language = "English",
volume = "12",
pages = "937--49",
journal = "Cerebellum",
issn = "1473-4222",
publisher = "Springer New York LLC",
number = "6",

}

RIS

TY - JOUR

T1 - Localization of CGRP receptor components, CGRP, and receptor binding sites in human and rhesus cerebellar cortex

AU - Eftekhari, Sajedeh

AU - Salvatore, Christopher A

AU - Gaspar, Renee C

AU - Roberts, Rhonda

AU - O'Malley, Stacey

AU - Zeng, Zhizhen

AU - Edvinsson, Lars

PY - 2013/12

Y1 - 2013/12

N2 - The cerebellum is classically considered to be mainly involved in motor processing, but studies have suggested several other functions, including pain processing. Calcitonin-gene-related peptide (CGRP) is a neuropeptide involved in migraine pathology, where there is elevated release of CGRP during migraine attacks and CGRP receptor antagonists have antimigraine efficacy. In the present study, we examined CGRP and CGRP receptor binding sites and protein expression in primate cerebellar cortex. Additionally, mRNA expression of the CGRP receptor components, calcitonin receptor-like receptor (CLR) and receptor activity modifying protein 1 (RAMP1), was examined. In addition, expression of procalcitonin was studied. We observed high [(3)H]MK-3207 (CGRP receptor antagonist) binding densities in the molecular layer of rhesus cerebellar cortex; however, due to the limit of resolution of the autoradiographic image the exact cellular localization could not be determined. Similarly, [(125)I]CGRP binding was observed in the molecular layer and Purkinje cell layer of human cerebellum. CLR and RAMP1 mRNA was expressed within the Purkinje cell layer and some expression was found in the molecular layer. Immunofluorescence revealed expression of CGRP, CLR, and RAMP1 in the Purkinje cells and in cells in the molecular layer. Procalcitonin was found in the same localization. Recent research in the biology of cerebellum indicates that it may have a role in nociception. For the first time we have identified CGRP and CGRP receptor binding sites together with CGRP receptor expression through protein and mRNA localization in primate cerebellar cortex. These results point toward a functional role of CGRP in cerebellum. Further efforts are needed to evaluate this.

AB - The cerebellum is classically considered to be mainly involved in motor processing, but studies have suggested several other functions, including pain processing. Calcitonin-gene-related peptide (CGRP) is a neuropeptide involved in migraine pathology, where there is elevated release of CGRP during migraine attacks and CGRP receptor antagonists have antimigraine efficacy. In the present study, we examined CGRP and CGRP receptor binding sites and protein expression in primate cerebellar cortex. Additionally, mRNA expression of the CGRP receptor components, calcitonin receptor-like receptor (CLR) and receptor activity modifying protein 1 (RAMP1), was examined. In addition, expression of procalcitonin was studied. We observed high [(3)H]MK-3207 (CGRP receptor antagonist) binding densities in the molecular layer of rhesus cerebellar cortex; however, due to the limit of resolution of the autoradiographic image the exact cellular localization could not be determined. Similarly, [(125)I]CGRP binding was observed in the molecular layer and Purkinje cell layer of human cerebellum. CLR and RAMP1 mRNA was expressed within the Purkinje cell layer and some expression was found in the molecular layer. Immunofluorescence revealed expression of CGRP, CLR, and RAMP1 in the Purkinje cells and in cells in the molecular layer. Procalcitonin was found in the same localization. Recent research in the biology of cerebellum indicates that it may have a role in nociception. For the first time we have identified CGRP and CGRP receptor binding sites together with CGRP receptor expression through protein and mRNA localization in primate cerebellar cortex. These results point toward a functional role of CGRP in cerebellum. Further efforts are needed to evaluate this.

KW - Aged

KW - Aged, 80 and over

KW - Animals

KW - Binding Sites

KW - Calcitonin Gene-Related Peptide

KW - Cerebellar Cortex

KW - Female

KW - Glutamate Decarboxylase

KW - Humans

KW - Macaca mulatta

KW - Male

KW - Nerve Tissue Proteins

KW - Postmortem Changes

KW - Protein Binding

KW - Radioligand Assay

KW - Receptors, Calcitonin Gene-Related Peptide

U2 - 10.1007/s12311-013-0509-4

DO - 10.1007/s12311-013-0509-4

M3 - Journal article

C2 - 23917876

VL - 12

SP - 937

EP - 949

JO - Cerebellum

JF - Cerebellum

SN - 1473-4222

IS - 6

ER -

ID: 44861387