Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

Local trauma in human patellar tendon leads to widespread changes in the tendon gene expression

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Physiological responses of human skeletal muscle to acute blood flow restricted exercise assessed by multimodal MRI

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Reply to Dutheil et al

    Publikation: Bidrag til tidsskriftKommentar/debatForskningpeer review

  3. Regional collagen turnover and composition of the human patellar tendon

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Menstrual cycle phase does not affect whole body peak fat oxidation rate during a graded exercise test

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  5. The influence of direct and indirect fibroblast cell contact on human myogenic cell behavior and gene expression in vitro

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. UTE T2* mapping of tendinopathic patellar tendons: an MRI reproducibility study

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Age-related myofiber atrophy in old mice is reversed by ten weeks voluntary high-resistance wheel running

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Insulin-stimulated glucose uptake partly relies on p21-activated kinase (PAK)2, but not PAK1, in mouse skeletal muscle

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. No detectable remodelling in adult human menisci: an analysis based on the C14 bomb pulse

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Vis graf over relationer

Low cellular activity and slow tissue turnover in human tendon may prolong resolution of tendinopathy. This may be stimulated by moderate localized traumas such as needle penetrations, but whether this results in a widespread cellular response in tendons is unknown. In an initial hypothesis-generating study, a trauma-induced tendon cell activity (increased total RNA and collagen I mRNA) was observed after repeated patellar tendon biopsies in young men. In a subsequent controlled study, 25 young men were treated with two 0.8-mm-diameter needle penetrations [n = 13, needle-group (NG)] or one 2.1-mm-diameter needle biopsy [n = 12, biopsy-group (BG)] in one patellar tendon. Four weeks later biopsies were taken from treated (5 mm lateral from trauma site) and contralateral tendons for analyses of RNA content (ribogreen assay), DNA content (PCR based), and gene expression for relevant target genes (Real-time RT-PCR) (NG, n = 11 and BG, n = 8). Intervention increased RNA content, and mRNA expression of collagen I and III and TGF-β1 (P < 0.05), with biopsy treatment having greatest effect (tendency for RNA and collagen I). Results for DNA content were inconclusive, and no changes were detected in expression of insulin-like growth factor-I, connective tissue growth factor, scleraxis, decorin, fibromodulin, tenascin-C, tenomodulin, VEGFa, CD68, IL-6, MMP12, and MMP13. In conclusion, a moderate trauma to a healthy human tendon (e.g., biopsy sampling) results in a widespread upregulation of tendon cell activity and their matrix protein expression. The findings have implications for design of studies on human tendon and may provide perspectives in future treatment strategies in tendinopathy.

OriginalsprogEngelsk
TidsskriftJournal of Applied Physiology Respiratory Environmental and Exercise Physiology
Vol/bind120
Udgave nummer9
Sider (fra-til)1000-10
Antal sider11
ISSN0161-7567
DOI
StatusUdgivet - 1 maj 2016

ID: 49587029