Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

Limited inter- and intra-patient sequence diversity of the genetic lineage A human metapneumovirus fusion gene

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Harvard

APA

CBE

MLA

Vancouver

Author

Bibtex

@article{87388a34434a4d08a98eb7c1f811f372,
title = "Limited inter- and intra-patient sequence diversity of the genetic lineage A human metapneumovirus fusion gene",
abstract = "Human metapneumovirus (hMPV) is associated with respiratory tract illness especially in young children. Two hMPV genetic lineages, A and B, and four sublineages A1, A2 and B1, B2 have been defined. Infection with hMPV occurs through membrane fusion mediated by the hMPV fusion (F) protein. In this study, the inter- and intra-patient genetic diversity of the lineage A hMPV F gene was investigated. Ten isolates were collected from 10 hMPV infected children. Viral RNA was isolated and amplified, and approximately 10 clones from each isolate were sequenced. Altogether 108 clones were successfully sequenced. The average interpatient sequence diversity was 1.68% and 1.64% at nucleotide and amino acid levels, respectively. The samples were divisible into two groups on the basis of intrapatient sequence diversity. In group 1 (4 children) the intra-patient sequence diversity was low (nt: 0.26-0.39%, aa: 0.51-0.94%) whereas group 2 (6 children) had a higher intra-patient sequence diversity (nt: 0.85-1.98%, aa: 1.08-2.22%). Phylogenetic analyses showed that the group 1 children harboured sublineage Al only, but interestingly group 2 children harboured both sublineages Al and A2, indicating they had been infected with at least two viruses. Several independent viruses contained premature stop codons in exactly identical positions resulting in truncated fusion proteins. Possibly this is a mechanism for immune system evasion. The F protein is a major antigenic determinant, and the limited sequence diversity observed lay emphasis on the hMPV F gene as a putative target for future vaccine development.",
keywords = "Amino Acid Sequence, Base Sequence, Child, Child, Preschool, Female, Genetic Variation, Humans, Infant, Male, Metapneumovirus, Molecular Sequence Data, Paramyxoviridae Infections, Phylogeny, Sequence Alignment, Viral Fusion Proteins",
author = "Winther, {Thilde Nordmann} and Madsen, {Chris D} and Anders Pedersen and {von Linstow}, Marie-Louise and Jesper Eugen-Olsen and Birthe Hogh",
year = "2005",
doi = "10.1007/s11262-005-2204-0",
language = "English",
volume = "31",
pages = "89--97",
journal = "Virus Genes",
issn = "0920-8569",
publisher = "Springer New York LLC",
number = "1",

}

RIS

TY - JOUR

T1 - Limited inter- and intra-patient sequence diversity of the genetic lineage A human metapneumovirus fusion gene

AU - Winther, Thilde Nordmann

AU - Madsen, Chris D

AU - Pedersen, Anders

AU - von Linstow, Marie-Louise

AU - Eugen-Olsen, Jesper

AU - Hogh, Birthe

PY - 2005

Y1 - 2005

N2 - Human metapneumovirus (hMPV) is associated with respiratory tract illness especially in young children. Two hMPV genetic lineages, A and B, and four sublineages A1, A2 and B1, B2 have been defined. Infection with hMPV occurs through membrane fusion mediated by the hMPV fusion (F) protein. In this study, the inter- and intra-patient genetic diversity of the lineage A hMPV F gene was investigated. Ten isolates were collected from 10 hMPV infected children. Viral RNA was isolated and amplified, and approximately 10 clones from each isolate were sequenced. Altogether 108 clones were successfully sequenced. The average interpatient sequence diversity was 1.68% and 1.64% at nucleotide and amino acid levels, respectively. The samples were divisible into two groups on the basis of intrapatient sequence diversity. In group 1 (4 children) the intra-patient sequence diversity was low (nt: 0.26-0.39%, aa: 0.51-0.94%) whereas group 2 (6 children) had a higher intra-patient sequence diversity (nt: 0.85-1.98%, aa: 1.08-2.22%). Phylogenetic analyses showed that the group 1 children harboured sublineage Al only, but interestingly group 2 children harboured both sublineages Al and A2, indicating they had been infected with at least two viruses. Several independent viruses contained premature stop codons in exactly identical positions resulting in truncated fusion proteins. Possibly this is a mechanism for immune system evasion. The F protein is a major antigenic determinant, and the limited sequence diversity observed lay emphasis on the hMPV F gene as a putative target for future vaccine development.

AB - Human metapneumovirus (hMPV) is associated with respiratory tract illness especially in young children. Two hMPV genetic lineages, A and B, and four sublineages A1, A2 and B1, B2 have been defined. Infection with hMPV occurs through membrane fusion mediated by the hMPV fusion (F) protein. In this study, the inter- and intra-patient genetic diversity of the lineage A hMPV F gene was investigated. Ten isolates were collected from 10 hMPV infected children. Viral RNA was isolated and amplified, and approximately 10 clones from each isolate were sequenced. Altogether 108 clones were successfully sequenced. The average interpatient sequence diversity was 1.68% and 1.64% at nucleotide and amino acid levels, respectively. The samples were divisible into two groups on the basis of intrapatient sequence diversity. In group 1 (4 children) the intra-patient sequence diversity was low (nt: 0.26-0.39%, aa: 0.51-0.94%) whereas group 2 (6 children) had a higher intra-patient sequence diversity (nt: 0.85-1.98%, aa: 1.08-2.22%). Phylogenetic analyses showed that the group 1 children harboured sublineage Al only, but interestingly group 2 children harboured both sublineages Al and A2, indicating they had been infected with at least two viruses. Several independent viruses contained premature stop codons in exactly identical positions resulting in truncated fusion proteins. Possibly this is a mechanism for immune system evasion. The F protein is a major antigenic determinant, and the limited sequence diversity observed lay emphasis on the hMPV F gene as a putative target for future vaccine development.

KW - Amino Acid Sequence

KW - Base Sequence

KW - Child

KW - Child, Preschool

KW - Female

KW - Genetic Variation

KW - Humans

KW - Infant

KW - Male

KW - Metapneumovirus

KW - Molecular Sequence Data

KW - Paramyxoviridae Infections

KW - Phylogeny

KW - Sequence Alignment

KW - Viral Fusion Proteins

U2 - 10.1007/s11262-005-2204-0

DO - 10.1007/s11262-005-2204-0

M3 - Journal article

C2 - 15965613

VL - 31

SP - 89

EP - 97

JO - Virus Genes

JF - Virus Genes

SN - 0920-8569

IS - 1

ER -

ID: 32577689