Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

Limited effects of preterm birth and the first enteral nutrition on cerebellum morphology and gene expression in piglets

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

DOI

  1. Increased oral sodium chloride intake in humans amplifies selectively postprandial GLP-1 but not GIP, CCK, and gastrin in plasma

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Changes in metabolism but not myocellular signaling by training with CHO-restriction in endurance athletes

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Four days of bed rest increases intrinsic mitochondrial respiratory capacity in young healthy males

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. High-intensity interval training changes mitochondrial respiratory capacity differently in adipose tissue and skeletal muscle

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  • Anders Bergström
  • Sanne S Kaalund
  • Kerstin Skovgaard
  • Anders D Andersen
  • Bente Pakkenberg
  • Ann Rosenørn
  • Ruurd M van Elburg
  • Thomas Thymann
  • Gorm O Greisen
  • Per T Sanglid
Vis graf over relationer

Preterm pigs show many signs of immaturity that are characteristic of preterm infants. In preterm infants, the cerebellum grows particularly rapid and hypoplasia and cellular lesions are associated with motor dysfunction and cognitive deficits. We hypothesized that functional brain delays observed in preterm pigs would be paralleled by both structural and molecular differences in the cerebellum relative to term born piglets. Cerebella were collected from term (n = 56) and preterm (90% gestation, n = 112) pigs at 0, 5, and 26 days after birth for stereological volume estimations, large-scale qPCR gene expression analyses (selected neurodevelopmental genes) and western blot protein expression analysis (Sonic Hedgehog pathway). Memory and learning was tested using a T-maze, documenting that preterm pigs showed delayed learning. Preterm pigs also showed reduced volume of both white and gray matter at all three ages but the proportion of white matter increased postnatally, relative to term pigs. Early initiation of enteral nutrition had limited structural or molecular effects. The Sonic Hedgehog pathway was unaffected by preterm birth. Few differences in expression of the selected genes were found, except consistently higher mRNA levels of Midkine, p75, and Neurotrophic factor 3 in the preterm cerebellum postnatally, probably reflecting an adaptive response to preterm birth. Pig cerebellar development appears more affected by postconceptional age than by environmental factors at birth or postnatally. Compensatory mechanisms following preterm birth may include faster white matter growth and increased expression of selected genes for neurotrophic factors and regulation of angiogenesis. While the pig cerebellum is immature in 90% gestation preterm pigs, it appears relatively mature and resilient toward environmental factors.

OriginalsprogEngelsk
TidsskriftPhysiological Reports
Vol/bind4
Udgave nummer14
DOI
StatusUdgivet - jul. 2016

ID: 49931906