Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

Leukocyte Activation Profile Assessed by Raman Spectroscopy Helps Diagnosing Infection and Sepsis

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Linking Stressful Life Events and Chronic Inflammation Using suPAR (Soluble Urokinase Plasminogen Activator Receptor)

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Effect of anakinra on mortality in patients with COVID-19: a systematic review and patient-level meta-analysis

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Circulating Osteopontin Levels and Outcomes in Patients Hospitalized for COVID-19

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. suPAR Cut-Offs for Risk Stratification in Patients With Symptoms of COVID-19

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  • Anuradha Ramoji
  • Daniel Thomas-Rüddel
  • Oleg Ryabchykov
  • Michael Bauer
  • Natalie Arend
  • Evangelos J Giamarellos-Bourboulis
  • Jesper Eugen-Olsen
  • Michael Kiehntopf
  • Thomas Bocklitz
  • Jürgen Popp
  • Frank Bloos
  • Ute Neugebauer
Vis graf over relationer

Objectives: Leukocytes are first responders to infection. Their activation state can reveal information about specific host immune response and identify dysregulation in sepsis. This study aims to use the Raman spectroscopic fingerprints of blood-derived leukocytes to differentiate inflammation, infection, and sepsis in hospitalized patients. Diagnostic sensitivity and specificity shall demonstrate the added value of the direct characterization of leukocyte's phenotype.

Design: Prospective nonrandomized, single-center, observational phase-II study (DRKS00006265).

Setting: Jena University Hospital, Germany.

Patients: Sixty-one hospitalized patients (19 with sterile inflammation, 23 with infection without organ dysfunction, 18 with sepsis according to Sepsis-3 definition).

Interventions: None (blood withdrawal).

Measurements AND MAIN RESULTS: Individual peripheral blood leukocytes were characterized by Raman spectroscopy. Reference diagnostics included established clinical scores, blood count, and biomarkers (C-reactive protein, procalcitonin and interleukin-6). Binary classification models using Raman data were able to distinguish patients with infection from patients without infection, as well as sepsis patients from patients without sepsis, with accuracies achieved with established biomarkers. Compared with biomarker information alone, an increase of 10% (to 93%) accuracy for the detection of infection and an increase of 18% (to 92%) for detection of sepsis were reached by adding the Raman information. Leukocytes from sepsis patients showed different Raman spectral features in comparison to the patients with infection that point to the special immune phenotype of sepsis patients.

Conclusions: Raman spectroscopy can extract information on leukocyte's activation state in a nondestructive, label-free manner to differentiate sterile inflammation, infection, and sepsis.

OriginalsprogEngelsk
Artikelnummere0394
TidsskriftCritical care explorations
Vol/bind3
Udgave nummer5
Sider (fra-til)1-11
Antal sider11
ISSN2639-8028
DOI
StatusUdgivet - 12 maj 2021

ID: 66132782