Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

LC-MS-Based Metabolomics Discovers Purine Endogenous Associations with Low-Dose Salbutamol in Urine Collected for Antidoping Tests

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Direct Monitoring of Exogenous γ-Hydroxybutyric Acid in Body Fluids by NMR Spectroscopy

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Electrochemical reduction of disulfide-containing proteins for hydrogen/deuterium exchange monitored by mass spectrometry

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Metabolic phenotype of the healthy rodent model using in-vial extraction of dried serum, urine, and cerebrospinal fluid spots

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Discovery and validation of plasma proteomic biomarkers relating to brain amyloid burden by SOMAscan assay

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Impact of binge drinking on hepatic lipid metabolism: lipidome analysis of liver vein and peripheral blood during acute alcohol intoxication

    Publikation: Bidrag til tidsskriftKonferenceabstrakt i tidsskriftForskningpeer review

  3. Polyols and Branched Chained Amino Acids Are Associated with Present and Future Renal Impairment in Type 1 Diabetes

    Publikation: Bidrag til tidsskriftKonferenceabstrakt i tidsskriftForskningpeer review

  4. High sensitivity LC-MS profiling of antibody-drug conjugates with difluoroacetic acid ion pairing

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Vis graf over relationer

Current antidoping analytical methods are tailored mainly to the targeting of known drugs and endogenous molecules. This causes difficulties in rapidly reacting to emerging threats, such as designer drugs, biological therapeutic agents, and technologies. Biomarkers are considered as a promising approach for the fight against these threats to sport. The main purpose of this study was to find surrogate biomarkers induced by the intake of small amounts of the model compound salbutamol and explore a sensitive approach to help screen for possible drug misuse. Urine samples (91) from athletes with detectable salbutamol (30) and negative samples (61) were analyzed using a UHPLC-MS. A third group (30) was created by spiking salbutamol into negative samples to eliminate confounding effects. Data were then analyzed in XCMS to extract metabolic features. Orthogonal partial least squares-discriminant analysis was performed to select features correlated with detectable salbutamol (p(corr) > 0.5) and ROC analysis was performed to measure the predictive potential of the markers. Univariate analysis including Mann-Whitney U test and Spearman's correlation was conducted on selected markers. A total of 7000 metabolic features were parsed, one feature identified as hypoxanthine increased with salbutamol (p < 0.001). The ROC curve of hypoxanthine returned an AUC of 0.79 (p < 0.001). Correlation with salbutamol (r = 0.415, p < 0.01, Spearman's correlation) showed hypoxanthine and purine metabolism have association with salbutamol administration. This surrogate discovery approach needs further PK studies but in the meantime can be used as an intelligence-based complementary approach for targeting of athletes to be further tested.

OriginalsprogEngelsk
TidsskriftAnalytical Chemistry
Vol/bind88
Udgave nummer4
Sider (fra-til)2243-2249
Antal sider7
ISSN0003-2700
DOI
StatusUdgivet - 16 feb. 2016
Eksternt udgivetJa

ID: 54953147