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Region Hovedstaden - en del af Københavns Universitetshospital
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Large-Scale mRNA Transfection of Dendritic Cells by Electroporation in Continuous Flow Systems

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Vis graf over relationer

Electroporation is well established for transient mRNA transfection of many mammalian cells, including immune cells such as dendritic cells used in cancer immunotherapy. Therapeutic application requires methods to efficiently electroporate and transfect millions of immune cells in a fast process with high cell survival. Continuous flow of suspended dendritic cells through a channel incorporating spatially separated microporous meshes with a synchronized electrical pulsing sequence can yield dendritic cell transfection rates of >75 % with survival rates of >90 %. This chapter describes the instrumentation and methods needed for the efficient transfection by electroporation of millions of dendritic cells in one continuous flow process.

OriginalsprogEngelsk
TidsskriftMethods in molecular biology
Vol/bind1428
Sider (fra-til)151-61
Antal sider11
ISSN1064-3745
DOI
StatusUdgivet - 2016

ID: 49640075