Lack of relationship between genotype and virulence in Candida species

Judith Díaz-García; Maiken C Arendrup; Rafael Cantón; Julio García-Rodríguez; Elia Gómez García de la Pedrosa; Gabriella Parisi; Javier Pemán; Brunella Posteraro; Maurizio Sanguinetti; Daniel Archimedes Da Matta; Arnaldo L Colombo; Patricia Muñoz; Carlos Sánchez-Carrillo; Jesús Guinea; Pilar Escribano

doi:10.1016/j.riam.2020.10.002

Lack of relationship between genotype and virulence in Candida species

Judith Díaz-García, Maiken C Arendrup, Rafael Cantón, Julio García-Rodríguez, Elia Gómez García de la Pedrosa, Gabriella Parisi, Javier Pemán, Brunella Posteraro, Maurizio Sanguinetti, Daniel Archimedes Da Matta, Arnaldo L Colombo, Patricia Muñoz, Carlos Sánchez-Carrillo, Jesús Guinea, Pilar Escribano

Afdeling for Klinisk Mikrobiologi

Abstrakt

BACKGROUND: The virulence of isolates among different Candida species causing candidemia may play a role in the prognosis of the patients. Furthermore, the potential relationship between genotype and virulence is still unclear and need to be further studied.

AIMS: We aim to assess the relationship between genotype and virulence in Candida species using a Galleria mellonella larvae infection model.

METHODS: One hundred and ninety-four isolates from 68 clusters (Candida albicans, 114/41; Candida parapsilosis, 74/24; Candida tropicalis, 6/3) were compared against the same number of each species singleton genotypes in terms of survival of G. mellonella larvae.

RESULTS: The median of survival and the IQR ranges of clusters and singleton were as follows: C. albicans (2 days, IQR 1.5-2 vs. 2 days, IQR 1-2.25), C. parapsilosis (2 days, IQR 1.5-2.6 vs. 2 days, IQR 2-3.3), and C. tropicalis (1 day, IQR 1-3.5 vs. 2 days, IQR 2-3.5; p<0.05). High intra-cluster variability in terms of median of survival was found regardless the species.

CONCLUSIONS: No relationship between genotype and virulence in Candida was observed with the G. mellonella model.

Originalsprog	Engelsk
Tidsskrift	Revista iberoamericana de micologia
Vol/bind	38
Udgave nummer	1
Sider (fra-til)	9-11
Antal sider	3
ISSN	1130-1406
DOI	https://doi.org/10.1016/j.riam.2020.10.002
Status	Udgivet - 29 dec. 2021

Emneord

Candida albicans
Candida parapsilosis
Candida tropicalis
Galleria mellonella
Genotyping
Virulence

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10.1016/j.riam.2020.10.002

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Citationsformater

Díaz-García, J., Arendrup, M. C., Cantón, R., García-Rodríguez, J., de la Pedrosa, E. G. G., Parisi, G., Pemán, J., Posteraro, B., Sanguinetti, M., Da Matta, D. A., Colombo, A. L., Muñoz, P., Sánchez-Carrillo, C., Guinea, J., & Escribano, P. (2021). Lack of relationship between genotype and virulence in Candida species. Revista iberoamericana de micologia, 38(1), 9-11. https://doi.org/10.1016/j.riam.2020.10.002

Díaz-García, J, Arendrup, MC, Cantón, R, García-Rodríguez, J, de la Pedrosa, EGG, Parisi, G, Pemán, J, Posteraro, B, Sanguinetti, M, Da Matta, DA, Colombo, AL, Muñoz, P, Sánchez-Carrillo, C, Guinea, J & Escribano, P 2021, 'Lack of relationship between genotype and virulence in Candida species', Revista iberoamericana de micologia, bind 38, nr. 1, s. 9-11. https://doi.org/10.1016/j.riam.2020.10.002

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title = "Lack of relationship between genotype and virulence in Candida species",

abstract = "BACKGROUND: The virulence of isolates among different Candida species causing candidemia may play a role in the prognosis of the patients. Furthermore, the potential relationship between genotype and virulence is still unclear and need to be further studied.AIMS: We aim to assess the relationship between genotype and virulence in Candida species using a Galleria mellonella larvae infection model.METHODS: One hundred and ninety-four isolates from 68 clusters (Candida albicans, 114/41; Candida parapsilosis, 74/24; Candida tropicalis, 6/3) were compared against the same number of each species singleton genotypes in terms of survival of G. mellonella larvae.RESULTS: The median of survival and the IQR ranges of clusters and singleton were as follows: C. albicans (2 days, IQR 1.5-2 vs. 2 days, IQR 1-2.25), C. parapsilosis (2 days, IQR 1.5-2.6 vs. 2 days, IQR 2-3.3), and C. tropicalis (1 day, IQR 1-3.5 vs. 2 days, IQR 2-3.5; p<0.05). High intra-cluster variability in terms of median of survival was found regardless the species.CONCLUSIONS: No relationship between genotype and virulence in Candida was observed with the G. mellonella model.",

keywords = "Candida parapsilosis, Candida/genetics, Candidemia, Genotype, Humans, Virulence/genetics, Candida albicans, Candida parapsilosis, Candida tropicalis, Galleria mellonella, Genotyping, Virulence",

author = "Judith D{\'i}az-Garc{\'i}a and Arendrup, {Maiken C} and Rafael Cant{\'o}n and Julio Garc{\'i}a-Rodr{\'i}guez and {de la Pedrosa}, {Elia G{\'o}mez Garc{\'i}a} and Gabriella Parisi and Javier Pem{\'a}n and Brunella Posteraro and Maurizio Sanguinetti and {Da Matta}, {Daniel Archimedes} and Colombo, {Arnaldo L} and Patricia Mu{\~n}oz and Carlos S{\'a}nchez-Carrillo and Jes{\'u}s Guinea and Pilar Escribano",

year = "2021",

month = dec,

day = "29",

doi = "10.1016/j.riam.2020.10.002",

language = "English",

volume = "38",

pages = "9--11",

journal = "Revista iberoamericana de micologia",

issn = "1130-1406",

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number = "1",

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T1 - Lack of relationship between genotype and virulence in Candida species

AU - Díaz-García, Judith

AU - Arendrup, Maiken C

AU - Cantón, Rafael

AU - García-Rodríguez, Julio

AU - de la Pedrosa, Elia Gómez García

AU - Parisi, Gabriella

AU - Pemán, Javier

AU - Posteraro, Brunella

AU - Sanguinetti, Maurizio

AU - Da Matta, Daniel Archimedes

AU - Colombo, Arnaldo L

AU - Muñoz, Patricia

AU - Sánchez-Carrillo, Carlos

AU - Guinea, Jesús

AU - Escribano, Pilar

PY - 2021/12/29

Y1 - 2021/12/29

N2 - BACKGROUND: The virulence of isolates among different Candida species causing candidemia may play a role in the prognosis of the patients. Furthermore, the potential relationship between genotype and virulence is still unclear and need to be further studied.AIMS: We aim to assess the relationship between genotype and virulence in Candida species using a Galleria mellonella larvae infection model.METHODS: One hundred and ninety-four isolates from 68 clusters (Candida albicans, 114/41; Candida parapsilosis, 74/24; Candida tropicalis, 6/3) were compared against the same number of each species singleton genotypes in terms of survival of G. mellonella larvae.RESULTS: The median of survival and the IQR ranges of clusters and singleton were as follows: C. albicans (2 days, IQR 1.5-2 vs. 2 days, IQR 1-2.25), C. parapsilosis (2 days, IQR 1.5-2.6 vs. 2 days, IQR 2-3.3), and C. tropicalis (1 day, IQR 1-3.5 vs. 2 days, IQR 2-3.5; p<0.05). High intra-cluster variability in terms of median of survival was found regardless the species.CONCLUSIONS: No relationship between genotype and virulence in Candida was observed with the G. mellonella model.

AB - BACKGROUND: The virulence of isolates among different Candida species causing candidemia may play a role in the prognosis of the patients. Furthermore, the potential relationship between genotype and virulence is still unclear and need to be further studied.AIMS: We aim to assess the relationship between genotype and virulence in Candida species using a Galleria mellonella larvae infection model.METHODS: One hundred and ninety-four isolates from 68 clusters (Candida albicans, 114/41; Candida parapsilosis, 74/24; Candida tropicalis, 6/3) were compared against the same number of each species singleton genotypes in terms of survival of G. mellonella larvae.RESULTS: The median of survival and the IQR ranges of clusters and singleton were as follows: C. albicans (2 days, IQR 1.5-2 vs. 2 days, IQR 1-2.25), C. parapsilosis (2 days, IQR 1.5-2.6 vs. 2 days, IQR 2-3.3), and C. tropicalis (1 day, IQR 1-3.5 vs. 2 days, IQR 2-3.5; p<0.05). High intra-cluster variability in terms of median of survival was found regardless the species.CONCLUSIONS: No relationship between genotype and virulence in Candida was observed with the G. mellonella model.

KW - Candida parapsilosis

KW - Candida/genetics

KW - Candidemia

KW - Genotype

KW - Humans

KW - Virulence/genetics

KW - Candida albicans

KW - Candida parapsilosis

KW - Candida tropicalis

KW - Galleria mellonella

KW - Genotyping

KW - Virulence

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U2 - 10.1016/j.riam.2020.10.002

DO - 10.1016/j.riam.2020.10.002

M3 - Journal article

C2 - 33358083

VL - 38

SP - 9

EP - 11

JO - Revista iberoamericana de micologia

JF - Revista iberoamericana de micologia

SN - 1130-1406

IS - 1

ER -

Lack of relationship between genotype and virulence in Candida species

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