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Joint sequencing of human and pathogen genomes reveals the genetics of pneumococcal meningitis

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Harvard

Lees, JA, Ferwerda, B, Kremer, PHC, Wheeler, NE, Serón, MV, Croucher, NJ, Gladstone, RA, Bootsma, HJ, Rots, NY, Wijmega-Monsuur, AJ, Sanders, EAM, Trzciński, K, Wyllie, AL, Zwinderman, AH, van den Berg, LH, van Rheenen, W, Veldink, JH, Harboe, ZB, Lundbo, LF, de Groot, LCPGM, van Schoor, NM, van der Velde, N, Ängquist, LH, Sørensen, TIA, Nohr, EA, Mentzer, AJ, Mills, TC, Knight, JC, du Plessis, M, Nzenze, S, Weiser, JN, Parkhill, J, Madhi, S, Benfield, T, von Gottberg, A, van der Ende, A, Brouwer, MC, Barrett, JC, Bentley, SD & van de Beek, D 2019, 'Joint sequencing of human and pathogen genomes reveals the genetics of pneumococcal meningitis' Nature Communications, bind 10, 2176. https://doi.org/10.1038/s41467-019-09976-3

APA

Lees, J. A., Ferwerda, B., Kremer, P. H. C., Wheeler, N. E., Serón, M. V., Croucher, N. J., ... van de Beek, D. (2019). Joint sequencing of human and pathogen genomes reveals the genetics of pneumococcal meningitis. Nature Communications, 10, [2176]. https://doi.org/10.1038/s41467-019-09976-3

CBE

Lees JA, Ferwerda B, Kremer PHC, Wheeler NE, Serón MV, Croucher NJ, Gladstone RA, Bootsma HJ, Rots NY, Wijmega-Monsuur AJ, Sanders EAM, Trzciński K, Wyllie AL, Zwinderman AH, van den Berg LH, van Rheenen W, Veldink JH, Harboe ZB, Lundbo LF, de Groot LCPGM, van Schoor NM, van der Velde N, Ängquist LH, Sørensen TIA, Nohr EA, Mentzer AJ, Mills TC, Knight JC, du Plessis M, Nzenze S, Weiser JN, Parkhill J, Madhi S, Benfield T, von Gottberg A, van der Ende A, Brouwer MC, Barrett JC, Bentley SD, van de Beek D. 2019. Joint sequencing of human and pathogen genomes reveals the genetics of pneumococcal meningitis. Nature Communications. 10. https://doi.org/10.1038/s41467-019-09976-3

MLA

Vancouver

Author

Lees, John A ; Ferwerda, Bart ; Kremer, Philip H C ; Wheeler, Nicole E ; Serón, Mercedes Valls ; Croucher, Nicholas J ; Gladstone, Rebecca A ; Bootsma, Hester J ; Rots, Nynke Y ; Wijmega-Monsuur, Alienke J ; Sanders, Elisabeth A M ; Trzciński, Krzysztof ; Wyllie, Anne L ; Zwinderman, Aeilko H ; van den Berg, Leonard H ; van Rheenen, Wouter ; Veldink, Jan H ; Harboe, Zitta B ; Lundbo, Lene F ; de Groot, Lisette C P G M ; van Schoor, Natasja M ; van der Velde, Nathalie ; Ängquist, Lars H ; Sørensen, Thorkild I A ; Nohr, Ellen A ; Mentzer, Alexander J ; Mills, Tara C ; Knight, Julian C ; du Plessis, Mignon ; Nzenze, Susan ; Weiser, Jeffrey N ; Parkhill, Julian ; Madhi, Shabir ; Benfield, Thomas ; von Gottberg, Anne ; van der Ende, Arie ; Brouwer, Matthijs C ; Barrett, Jeffrey C ; Bentley, Stephen D ; van de Beek, Diederik. / Joint sequencing of human and pathogen genomes reveals the genetics of pneumococcal meningitis. I: Nature Communications. 2019 ; Bind 10.

Bibtex

@article{4b4c74f11a754a90afaa4f88fd554778,
title = "Joint sequencing of human and pathogen genomes reveals the genetics of pneumococcal meningitis",
abstract = "Streptococcus pneumoniae is a common nasopharyngeal colonizer, but can also cause life-threatening invasive diseases such as empyema, bacteremia and meningitis. Genetic variation of host and pathogen is known to play a role in invasive pneumococcal disease, though to what extent is unknown. In a genome-wide association study of human and pathogen we show that human variation explains almost half of variation in susceptibility to pneumococcal meningitis and one-third of variation in severity, identifying variants in CCDC33 associated with susceptibility. Pneumococcal genetic variation explains a large amount of invasive potential (70{\%}), but has no effect on severity. Serotype alone is insufficient to explain invasiveness, suggesting other pneumococcal factors are involved in progression to invasive disease. We identify pneumococcal genes involved in invasiveness including pspC and zmpD, and perform a human-bacteria interaction analysis. These genes are potential candidates for the development of more broadly-acting pneumococcal vaccines.",
author = "Lees, {John A} and Bart Ferwerda and Kremer, {Philip H C} and Wheeler, {Nicole E} and Ser{\'o}n, {Mercedes Valls} and Croucher, {Nicholas J} and Gladstone, {Rebecca A} and Bootsma, {Hester J} and Rots, {Nynke Y} and Wijmega-Monsuur, {Alienke J} and Sanders, {Elisabeth A M} and Krzysztof Trzciński and Wyllie, {Anne L} and Zwinderman, {Aeilko H} and {van den Berg}, {Leonard H} and {van Rheenen}, Wouter and Veldink, {Jan H} and Harboe, {Zitta B} and Lundbo, {Lene F} and {de Groot}, {Lisette C P G M} and {van Schoor}, {Natasja M} and {van der Velde}, Nathalie and {\"A}ngquist, {Lars H} and S{\o}rensen, {Thorkild I A} and Nohr, {Ellen A} and Mentzer, {Alexander J} and Mills, {Tara C} and Knight, {Julian C} and {du Plessis}, Mignon and Susan Nzenze and Weiser, {Jeffrey N} and Julian Parkhill and Shabir Madhi and Thomas Benfield and {von Gottberg}, Anne and {van der Ende}, Arie and Brouwer, {Matthijs C} and Barrett, {Jeffrey C} and Bentley, {Stephen D} and {van de Beek}, Diederik",
year = "2019",
month = "5",
day = "15",
doi = "10.1038/s41467-019-09976-3",
language = "English",
volume = "10",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",

}

RIS

TY - JOUR

T1 - Joint sequencing of human and pathogen genomes reveals the genetics of pneumococcal meningitis

AU - Lees, John A

AU - Ferwerda, Bart

AU - Kremer, Philip H C

AU - Wheeler, Nicole E

AU - Serón, Mercedes Valls

AU - Croucher, Nicholas J

AU - Gladstone, Rebecca A

AU - Bootsma, Hester J

AU - Rots, Nynke Y

AU - Wijmega-Monsuur, Alienke J

AU - Sanders, Elisabeth A M

AU - Trzciński, Krzysztof

AU - Wyllie, Anne L

AU - Zwinderman, Aeilko H

AU - van den Berg, Leonard H

AU - van Rheenen, Wouter

AU - Veldink, Jan H

AU - Harboe, Zitta B

AU - Lundbo, Lene F

AU - de Groot, Lisette C P G M

AU - van Schoor, Natasja M

AU - van der Velde, Nathalie

AU - Ängquist, Lars H

AU - Sørensen, Thorkild I A

AU - Nohr, Ellen A

AU - Mentzer, Alexander J

AU - Mills, Tara C

AU - Knight, Julian C

AU - du Plessis, Mignon

AU - Nzenze, Susan

AU - Weiser, Jeffrey N

AU - Parkhill, Julian

AU - Madhi, Shabir

AU - Benfield, Thomas

AU - von Gottberg, Anne

AU - van der Ende, Arie

AU - Brouwer, Matthijs C

AU - Barrett, Jeffrey C

AU - Bentley, Stephen D

AU - van de Beek, Diederik

PY - 2019/5/15

Y1 - 2019/5/15

N2 - Streptococcus pneumoniae is a common nasopharyngeal colonizer, but can also cause life-threatening invasive diseases such as empyema, bacteremia and meningitis. Genetic variation of host and pathogen is known to play a role in invasive pneumococcal disease, though to what extent is unknown. In a genome-wide association study of human and pathogen we show that human variation explains almost half of variation in susceptibility to pneumococcal meningitis and one-third of variation in severity, identifying variants in CCDC33 associated with susceptibility. Pneumococcal genetic variation explains a large amount of invasive potential (70%), but has no effect on severity. Serotype alone is insufficient to explain invasiveness, suggesting other pneumococcal factors are involved in progression to invasive disease. We identify pneumococcal genes involved in invasiveness including pspC and zmpD, and perform a human-bacteria interaction analysis. These genes are potential candidates for the development of more broadly-acting pneumococcal vaccines.

AB - Streptococcus pneumoniae is a common nasopharyngeal colonizer, but can also cause life-threatening invasive diseases such as empyema, bacteremia and meningitis. Genetic variation of host and pathogen is known to play a role in invasive pneumococcal disease, though to what extent is unknown. In a genome-wide association study of human and pathogen we show that human variation explains almost half of variation in susceptibility to pneumococcal meningitis and one-third of variation in severity, identifying variants in CCDC33 associated with susceptibility. Pneumococcal genetic variation explains a large amount of invasive potential (70%), but has no effect on severity. Serotype alone is insufficient to explain invasiveness, suggesting other pneumococcal factors are involved in progression to invasive disease. We identify pneumococcal genes involved in invasiveness including pspC and zmpD, and perform a human-bacteria interaction analysis. These genes are potential candidates for the development of more broadly-acting pneumococcal vaccines.

UR - http://www.scopus.com/inward/record.url?scp=85065780769&partnerID=8YFLogxK

U2 - 10.1038/s41467-019-09976-3

DO - 10.1038/s41467-019-09976-3

M3 - Journal article

VL - 10

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

M1 - 2176

ER -

ID: 57193799