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Isolation of RNP granules
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review
Harvard
Jønson, L, Nielsen, FC & Christiansen, J 2011, 'Isolation of RNP granules' Methods in Molecular Biology, bind 703, s. 265-73. https://doi.org/10.1007/978-1-59745-248-9_18
APA
Jønson, L., Nielsen, F. C., & Christiansen, J. (2011). Isolation of RNP granules. Methods in Molecular Biology, 703, 265-73. https://doi.org/10.1007/978-1-59745-248-9_18
CBE
Jønson L, Nielsen FC, Christiansen J. 2011. Isolation of RNP granules. Methods in Molecular Biology. 703:265-73. https://doi.org/10.1007/978-1-59745-248-9_18
MLA
Jønson, Lars, Finn Cilius Nielsen og Jan Christiansen. "Isolation of RNP granules". Methods in Molecular Biology. 2011, 703. 265-73. https://doi.org/10.1007/978-1-59745-248-9_18
Vancouver
Jønson L, Nielsen FC, Christiansen J. Isolation of RNP granules. Methods in Molecular Biology. 2011;703:265-73. https://doi.org/10.1007/978-1-59745-248-9_18
Author
Bibtex
@article{4fd982dd322447eaa7b0f2ee87bc3220,
title = "Isolation of RNP granules",
abstract = "The post-transcriptional operon provides a means of synexpression of mRNAs encoding interrelated proteins. The coordination of gene expression may be achieved by a trans-acting RNA-binding protein attaching to similar cis-elements in different, yet functionally clustered, mRNAs. The RNP granule can be regarded as a supramolecular assembly of RNA and protein, probably representing several overlapping post-transcriptional operons. The present protocol describes how RNP granules may be isolated by the transgenic expression of a 3X FLAG version of an RNA-binding protein under tetracycline control via the tetracycline receptor/operator complex. In this way, inclusion of an appropriate tetracycline concentration ensures expression of the tagged version at the endogenous level, and the 3X FLAG tag is a convenient {"}handle{"} for the subsequent immunoprecipitation by immobilized anti-FLAG antibody.",
keywords = "Blotting, Western, Cytoplasmic Granules, Electrophoresis, Polyacrylamide Gel, Gene Expression Regulation, Immunoprecipitation, RNA, Messenger, RNA-Binding Proteins, Ribonucleoproteins, Tetracycline",
author = "Lars J{\o}nson and Nielsen, {Finn Cilius} and Jan Christiansen",
year = "2011",
doi = "10.1007/978-1-59745-248-9_18",
language = "English",
volume = "703",
pages = "265--73",
journal = "Methods in Molecular Biology",
issn = "1064-3745",
publisher = "Humana Press, Inc",
}
RIS
TY - JOUR
T1 - Isolation of RNP granules
AU - Jønson, Lars
AU - Nielsen, Finn Cilius
AU - Christiansen, Jan
PY - 2011
Y1 - 2011
N2 - The post-transcriptional operon provides a means of synexpression of mRNAs encoding interrelated proteins. The coordination of gene expression may be achieved by a trans-acting RNA-binding protein attaching to similar cis-elements in different, yet functionally clustered, mRNAs. The RNP granule can be regarded as a supramolecular assembly of RNA and protein, probably representing several overlapping post-transcriptional operons. The present protocol describes how RNP granules may be isolated by the transgenic expression of a 3X FLAG version of an RNA-binding protein under tetracycline control via the tetracycline receptor/operator complex. In this way, inclusion of an appropriate tetracycline concentration ensures expression of the tagged version at the endogenous level, and the 3X FLAG tag is a convenient "handle" for the subsequent immunoprecipitation by immobilized anti-FLAG antibody.
AB - The post-transcriptional operon provides a means of synexpression of mRNAs encoding interrelated proteins. The coordination of gene expression may be achieved by a trans-acting RNA-binding protein attaching to similar cis-elements in different, yet functionally clustered, mRNAs. The RNP granule can be regarded as a supramolecular assembly of RNA and protein, probably representing several overlapping post-transcriptional operons. The present protocol describes how RNP granules may be isolated by the transgenic expression of a 3X FLAG version of an RNA-binding protein under tetracycline control via the tetracycline receptor/operator complex. In this way, inclusion of an appropriate tetracycline concentration ensures expression of the tagged version at the endogenous level, and the 3X FLAG tag is a convenient "handle" for the subsequent immunoprecipitation by immobilized anti-FLAG antibody.
KW - Blotting, Western
KW - Cytoplasmic Granules
KW - Electrophoresis, Polyacrylamide Gel
KW - Gene Expression Regulation
KW - Immunoprecipitation
KW - RNA, Messenger
KW - RNA-Binding Proteins
KW - Ribonucleoproteins
KW - Tetracycline
U2 - 10.1007/978-1-59745-248-9_18
DO - 10.1007/978-1-59745-248-9_18
M3 - Journal article
VL - 703
SP - 265
EP - 273
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
SN - 1064-3745
ER -
ID: 33166053