Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

Isolation and expression of the Pneumocystis carinii thymidylate synthase gene

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Unfolding of monomeric lipoprotein lipase by ANGPTL4: Insight into the regulation of plasma triglyceride metabolism

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Galnt11 regulates kidney function by glycosylating the endocytosis receptor megalin to modulate ligand binding

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. N-acyl taurines are endogenous lipid messengers that improve glucose homeostasis

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Early sarcomere and metabolic defects in a zebrafish pitx2c cardiac arrhythmia model

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  5. Mode of action of quinoline antimalarial drugs in red blood cells infected by Plasmodium falciparum revealed in vivo

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Profound Endothelial Damage Predicts Impending Organ Failure and Death in Sepsis

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. The Danish Microbiology Database (MiBa) 2010 to 2013

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Vis graf over relationer
The thymidylate synthase (TS) gene from Pneumocystis carinii has been isolated from complementary and genomic DNA libraries and expressed in Escherichia coli. The coding sequence of TS is 891 nucleotides, encoding a 297-amino acid protein of Mr 34,269. The deduced amino acid sequence is similar to TS from other organisms and is most closely related to the enzyme from Saccharomyces cerevisiae with 65% identity. TS is found on a 330-kilobase-pair chromosome in P. carinii. While TS and dihydrofolate reductase reside on a single polypeptide chain in all protozoa studied to date, TS is not linked to dihydrofolate reductase in P. carinii. The TS gene shows the presence of four small intervening sequences, some of which interrupt the coding sequence in highly ordered structural regions of the protein. Heterologous expression of P. carinii TS in E. coli was accomplished by cloning the coding sequence into plasmid vectors under control of the lac and tac promoters. These constructs direct the synthesis of catalytically active enzyme to the extent of 2% of total soluble protein.
OriginalsprogEngelsk
TidsskriftProceedings of the National Academy of Sciences of the United States of America
Vol/bind86
Udgave nummer17
Sider (fra-til)6503-7
Antal sider5
ISSN0027-8424
StatusUdgivet - sep. 1989

ID: 39873641