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Isolation and Characterization of Human Brown Adipocytes

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Vis graf over relationer

Brown adipose tissue (BAT) is a thermoregulatory fat with energy-consuming properties. The location and heterogeneity of this tissue makes it complicated to sample before and after interventions in humans, and an in vitro model for mechanistic and molecular studies is therefore of great value. We here describe a protocol for isolation of progenitors from the stromal vascular fraction of BAT biopsies obtained surgically from adult humans. We further present how these cells are differentiated in vitro and finally how they are characterized for thermogenic capacity. Methods for characterization described here include norepinephrine-induced thermogenic gene expression using qPCR; norepinephrine-induced mitochondrial uncoupling using the Seahorse XFe96 Analyzer, and norepinephrine-induced expression of UCP1 using the RNAscope® Technology.

OriginalsprogEngelsk
TidsskriftMethods in Molecular Biology
Vol/bind2448
Sider (fra-til)217-234
Antal sider18
ISSN1064-3745
DOI
StatusUdgivet - 2022

Bibliografisk note

© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

ID: 74731197