Ischemic heart disease down-regulates angiotensin type 1 receptor mRNA in human coronary arteries

Angelica Wackenfors; Malin Emilson; Richard Ingemansson; Lars Edvinsson; Malin Malmsjö

doi:10.1016/j.ejphar.2004.09.039

Ischemic heart disease down-regulates angiotensin type 1 receptor mRNA in human coronary arteries

Angelica Wackenfors, Malin Emilson, Richard Ingemansson, Lars Edvinsson, Malin Malmsjö

8 Citationer (Scopus)

Abstract

Angiotensin II is important in the development of cardiovascular disease. In the present study, angiotensin II receptor mRNA levels were quantified by real-time polymerase chain reaction (real-time PCR) in human coronary arteries from patients with ischemic heart disease and controls. Furthermore, the suitability of artery culture for studying angiotensin receptor changes was evaluated by in vitro pharmacology and real-time PCR. The angiotensin type 1 (AT1) receptor mRNA levels were down-regulated in human coronary arteries from patients with ischemic heart disease as compared to controls (P<0.05). Culture of coronary arteries for 48 h induced down-regulation of the angiotensin AT1 and AT2 receptor mRNA levels and also a less efficacious angiotensin II-induced vasoconstriction (Emax=103+/-2% before and 23+/-7% after artery culture, P<0.001). Artery culture may thus be a suitable method for studying angiotensin receptor regulation.

Originalsprog	Engelsk
Tidsskrift	European Journal of Pharmacology
Vol/bind	503
Udgave nummer	1-3
Sider (fra-til)	147-53
Antal sider	7
ISSN	0014-2999
DOI	https://doi.org/10.1016/j.ejphar.2004.09.039
Status	Udgivet - 25 okt. 2004
Udgivet eksternt	Ja

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10.1016/j.ejphar.2004.09.039

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@article{ed01ade8a61c49ce96e4369a46009a70,

title = "Ischemic heart disease down-regulates angiotensin type 1 receptor mRNA in human coronary arteries",

abstract = "Angiotensin II is important in the development of cardiovascular disease. In the present study, angiotensin II receptor mRNA levels were quantified by real-time polymerase chain reaction (real-time PCR) in human coronary arteries from patients with ischemic heart disease and controls. Furthermore, the suitability of artery culture for studying angiotensin receptor changes was evaluated by in vitro pharmacology and real-time PCR. The angiotensin type 1 (AT1) receptor mRNA levels were down-regulated in human coronary arteries from patients with ischemic heart disease as compared to controls (P<0.05). Culture of coronary arteries for 48 h induced down-regulation of the angiotensin AT1 and AT2 receptor mRNA levels and also a less efficacious angiotensin II-induced vasoconstriction (Emax=103+/-2% before and 23+/-7% after artery culture, P<0.001). Artery culture may thus be a suitable method for studying angiotensin receptor regulation.",

keywords = "Cells, Cultured, Coronary Vessels, Down-Regulation, Endothelium, Vascular, Humans, Muscle Contraction, Muscle, Smooth, Vascular, Myocardial Ischemia, Organ Culture Techniques, Potassium, RNA, Messenger, Receptor, Angiotensin, Type 1, Receptor, Angiotensin, Type 2, Reverse Transcriptase Polymerase Chain Reaction, Vasoconstriction",

author = "Angelica Wackenfors and Malin Emilson and Richard Ingemansson and Lars Edvinsson and Malin Malmsj{\"o}",

year = "2004",

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doi = "10.1016/j.ejphar.2004.09.039",

language = "English",

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TY - JOUR

T1 - Ischemic heart disease down-regulates angiotensin type 1 receptor mRNA in human coronary arteries

AU - Wackenfors, Angelica

AU - Emilson, Malin

AU - Ingemansson, Richard

AU - Edvinsson, Lars

AU - Malmsjö, Malin

PY - 2004/10/25

Y1 - 2004/10/25

N2 - Angiotensin II is important in the development of cardiovascular disease. In the present study, angiotensin II receptor mRNA levels were quantified by real-time polymerase chain reaction (real-time PCR) in human coronary arteries from patients with ischemic heart disease and controls. Furthermore, the suitability of artery culture for studying angiotensin receptor changes was evaluated by in vitro pharmacology and real-time PCR. The angiotensin type 1 (AT1) receptor mRNA levels were down-regulated in human coronary arteries from patients with ischemic heart disease as compared to controls (P<0.05). Culture of coronary arteries for 48 h induced down-regulation of the angiotensin AT1 and AT2 receptor mRNA levels and also a less efficacious angiotensin II-induced vasoconstriction (Emax=103+/-2% before and 23+/-7% after artery culture, P<0.001). Artery culture may thus be a suitable method for studying angiotensin receptor regulation.

AB - Angiotensin II is important in the development of cardiovascular disease. In the present study, angiotensin II receptor mRNA levels were quantified by real-time polymerase chain reaction (real-time PCR) in human coronary arteries from patients with ischemic heart disease and controls. Furthermore, the suitability of artery culture for studying angiotensin receptor changes was evaluated by in vitro pharmacology and real-time PCR. The angiotensin type 1 (AT1) receptor mRNA levels were down-regulated in human coronary arteries from patients with ischemic heart disease as compared to controls (P<0.05). Culture of coronary arteries for 48 h induced down-regulation of the angiotensin AT1 and AT2 receptor mRNA levels and also a less efficacious angiotensin II-induced vasoconstriction (Emax=103+/-2% before and 23+/-7% after artery culture, P<0.001). Artery culture may thus be a suitable method for studying angiotensin receptor regulation.

KW - Cells, Cultured

KW - Coronary Vessels

KW - Down-Regulation

KW - Endothelium, Vascular

KW - Humans

KW - Muscle Contraction

KW - Muscle, Smooth, Vascular

KW - Myocardial Ischemia

KW - Organ Culture Techniques

KW - Potassium

KW - RNA, Messenger

KW - Receptor, Angiotensin, Type 1

KW - Receptor, Angiotensin, Type 2

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Vasoconstriction

U2 - 10.1016/j.ejphar.2004.09.039

DO - 10.1016/j.ejphar.2004.09.039

M3 - Journal article

C2 - 15496309

SN - 0014-2999

VL - 503

SP - 147

EP - 153

JO - European Journal of Pharmacology

JF - European Journal of Pharmacology

IS - 1-3

ER -

Ischemic heart disease down-regulates angiotensin type 1 receptor mRNA in human coronary arteries

Abstract

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