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Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

Iron induced RNA-oxidation in the general population and in mouse tissue

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Urinary markers of nucleic acid oxidation increase with age, obesity and insulin resistance in Danish children and adolescents

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Oxidatively generated modifications to nucleic acids in vivo: Measurement in urine and plasma

    Publikation: Bidrag til tidsskriftReviewForskningpeer review

  3. The Effect of Different Training Intensities on Oxidatively Generated Modifications of Nucleic Acids: A Randomized, Controlled Trial

    Publikation: Bidrag til tidsskriftKonferenceabstrakt i tidsskriftForskningpeer review

Vis graf over relationer

Iron promotes formation of hydroxyl radicals by the Fenton reaction, subsequently leading to potential oxidatively generated damage of nucleic acids. Oxidatively generated damage to RNA, measured as 8-oxo-7,8-dihydroguanosine (8-oxoGuo) in urine, is increased in patients with genetic iron overload, which have led us to test the hypothesis that high iron status, assessed by iron biomarkers and genetic disposition, increases urinary excretion of 8-oxoGuo. In a general Danish population study we used a Mendelian randomization design with HFE genotypes as a proxy for iron status and supplemented with ex vivo experiments in mice muscle tissue exposed to iron(II) sulfate to attempt to clarify this hypothesis. The biomarkers ferritin, transferrin, and transferrin saturation (TS) were associated with 8-oxoGuo (in linear univariable and multivariable regression analyses: P < 0.001). Mendelian randomization indicated a causal pathway between genetically elevated iron biomarkers (assessed by ferritin and TS) and high levels of 8-oxoGuo. The ex vivo experiments showed a monotonically increase in 8-oxoGuo with increased iron concentration (ANOVA: P = 0.0008) that was prevented with iron chelation (P = 0.01). Our results indicate a causal relationship between iron biomarkers and 8-oxoGuo. Furthermore, the ex vivo experiment shows a mechanistic link between iron and 8-oxoGuo formation. Both iron overload and the biomarker 8-oxoGuo have been linked to e.g. diabetes, which merits future studies to investigate if iron induced 8-oxoGuo is involved in disease development.

OriginalsprogEngelsk
TidsskriftFree Radical Biology and Medicine
Vol/bind115
Sider (fra-til)127-135
Antal sider9
ISSN0891-5849
DOI
StatusUdgivet - 2018

ID: 52211235