Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

Investigation of the introduction and dissemination of vanB Enterococcus faecium in the Capital Region of Denmark and development of a rapid and accurate clone-specific vanB E. faecium PCR

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

DOI

  1. ResFinder 4.0 for predictions of phenotypes from genotypes

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. EUCAST rapid antimicrobial susceptibility testing (RAST) in blood cultures: Validation in 55 european laboratories

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Staphylococcus saprophyticus Causing Infections in Humans Is Associated with High Resistance to Heavy Metals

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Staphylococcus saprophyticus From Clinical and Environmental Origins Have Distinct Biofilm Composition

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Comparison of 16 Serological SARS-CoV-2 Immunoassays in 16 Clinical Laboratories

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Vis graf over relationer

BACKGROUND: During 2018-19, an increase of vanB vancomycin-resistant Enterococcus faecium (VREfm) was observed in the Capital Region of Denmark. vanA/vanB PCR performed directly on rectal swabs is accurate in detection of vanA; however, the positive predictive value for vanB-positive samples is low because of the presence of vanB in non-enterococcal gut commensals.

OBJECTIVES: We investigated the epidemiology and clonal relatedness of vanB VREfm from the period 2015-19 and describe the application of a clone-specific vanB VREfm PCR assay for rapid and accurate detection of vanB VREfm in rectal screening samples.

METHODS: vanB VREfm were investigated using epidemiological data and WGS data. The SeqSphere+ software was used to analyse MLST and cgMLST, and de novo assemblies were annotated to determine insertion sites for the vanB transposon (Tn1549). A clone-specific vanB VREfm PCR assay was designed to detect the sequence bridging Tn1549 and the E. faecium chromosome (araA2) in the dominant cluster.

RESULTS: Two hundred and seventy-five vanB VREfm isolates were identified, of which 76% were identified in 2019. A dominant cluster (Cluster 1, n = 204, 74%), six minor clusters and 15 singletons were identified. All Cluster 1 isolates and six non-Cluster 1 isolates had Tn1549 integrated into araA2. In 2019, the PCR assay would have detected 92% of all rectal screening samples containing vanB VREfm.

CONCLUSIONS: vanB VREfm increased due to the introduction and nosocomial transmission of the successful Cluster 1. The clone-specific PCR assay detected vanB VREfm outbreak isolates in rectal screening samples rapidly and accurately.

OriginalsprogEngelsk
TidsskriftThe Journal of antimicrobial chemotherapy
Vol/bind76
Udgave nummer9
Sider (fra-til)2260-2267
Antal sider8
ISSN0305-7453
DOI
StatusUdgivet - sep. 2021

ID: 66421050