TY - JOUR
T1 - Intramyocellular triglyceride content in man, influence of sex, obesity and glycaemic control
AU - Haugaard, Steen
AU - Mu, Huiling
AU - Vaag, Allan
AU - Madsbad, Sten
PY - 2009
Y1 - 2009
N2 - Objective. It remains unknown whether sex impacts on intramyocellular triglyceride (IMTG) in obesity, as has been shown in non-obese subjects, and, if so, whether this may imply on the association between IMTG and insulin sensitivity. Subjects and methods. A muscle biopsy from vastus lateralis was obtained in 27 obese women (BMI=35.5+/-0.8kg/m2, mean+/-SEM, percentage of body fat, PBF=44+/-1, n=7 impaired-fasting-glucose, n=7 type2 diabetes), 20 obese men (BMI=35.8+/-0.8 kg/m2; PBF=33+/-1, n=4 impaired-fasting-glucose; n=6 type2 diabetes) and 12 lean sedentary healthy individuals (controls; n=7 women, BMI=21.8 kg/m2, PBF=20+/-2; n=5 men, BMI=23.6 kg/m2, PBF=13+/-2). IMTG was determined by chromatography. Results. IMTG was increased 2-fold in obese women compared to obese men, lean men, and lean women, respectively (21.9+/-2.4mg/g wet weight, 10.9+/-1.5mg/g, 9.8+/-2.1mg/g and 10.9+/-2.4mg/g, P<0.001). Among obese subjects of either gender IMTG did not increase along with reduced glycaemic control in terms of impaired-fasting-glucose and diabetes. Plasma insulin levels, which were similar among obese women with different glycaemic control levels, but much lower in lean women, paralleled the changes in IMTG among women. PBF was associated with IMTG in all subjects (P<0.001). In a linear model sex (P<0.05) and PBF (P<0.05) independently explained variation in IMTG. Plasma free fatty acids correlated with IMTG in all subjects (P<0.005). Conclusion. Obese women display twice as much IMTG as obese men matched for BMI. Increased IMTG could be a pathophysiological element or a mere physiological phenomenon in feminine obesity ensuing prior to impaired glycaemic control, but associated with increased body fat, circulating free fatty acids and insulin.
AB - Objective. It remains unknown whether sex impacts on intramyocellular triglyceride (IMTG) in obesity, as has been shown in non-obese subjects, and, if so, whether this may imply on the association between IMTG and insulin sensitivity. Subjects and methods. A muscle biopsy from vastus lateralis was obtained in 27 obese women (BMI=35.5+/-0.8kg/m2, mean+/-SEM, percentage of body fat, PBF=44+/-1, n=7 impaired-fasting-glucose, n=7 type2 diabetes), 20 obese men (BMI=35.8+/-0.8 kg/m2; PBF=33+/-1, n=4 impaired-fasting-glucose; n=6 type2 diabetes) and 12 lean sedentary healthy individuals (controls; n=7 women, BMI=21.8 kg/m2, PBF=20+/-2; n=5 men, BMI=23.6 kg/m2, PBF=13+/-2). IMTG was determined by chromatography. Results. IMTG was increased 2-fold in obese women compared to obese men, lean men, and lean women, respectively (21.9+/-2.4mg/g wet weight, 10.9+/-1.5mg/g, 9.8+/-2.1mg/g and 10.9+/-2.4mg/g, P<0.001). Among obese subjects of either gender IMTG did not increase along with reduced glycaemic control in terms of impaired-fasting-glucose and diabetes. Plasma insulin levels, which were similar among obese women with different glycaemic control levels, but much lower in lean women, paralleled the changes in IMTG among women. PBF was associated with IMTG in all subjects (P<0.001). In a linear model sex (P<0.05) and PBF (P<0.05) independently explained variation in IMTG. Plasma free fatty acids correlated with IMTG in all subjects (P<0.005). Conclusion. Obese women display twice as much IMTG as obese men matched for BMI. Increased IMTG could be a pathophysiological element or a mere physiological phenomenon in feminine obesity ensuing prior to impaired glycaemic control, but associated with increased body fat, circulating free fatty acids and insulin.
U2 - 10.1530/EJE-08-0931
DO - 10.1530/EJE-08-0931
M3 - Journal article
C2 - 19417077
SN - 0804-4643
VL - 161
SP - 57
EP - 64
JO - European journal of endocrinology / European Federation of Endocrine Societies
JF - European journal of endocrinology / European Federation of Endocrine Societies
IS - 1
ER -