TY - JOUR
T1 - Interleukin-1 affects the function of cultured human thyroid cells
AU - Krogh Rasmussen, A
AU - Bech, K
AU - Feldt-Rasmussen, U
AU - Poulsen, S
AU - Holten, I
AU - Ryberg, M
AU - Dinarello, C A
AU - Siersbaek-Nielsen, K
AU - Friis, T
AU - Bendtzen, K
PY - 1988/8
Y1 - 1988/8
N2 - Cytokines are peptide hormones essential for cellular communication in the immune response. The purpose of this study was to investigate the influence of cytokines, especially recombinant interleukin 1 beta (rIL-1 beta), on human thyroid cells. Thyroglobulin (Tg) was measured by a double antibody radioimmunoassay, and cyclic AMP (cAMP) by a competitive protein binding assay. Supernatants from unstimulated and phytohaemagglutinin-stimulated blood mononuclear cells were added to human thyroid cells cultured in monolayers. A dose-dependent inhibition of the secretion of Tg and cAMP was demonstrated. Both subcultured and primary cultured cells incubated with rIL-1 beta at pharmacological levels (10(-1)-10(2) U/ml) exhibited an inhibition of Tg and cAMP secretion, while at physiological levels (10(-5)-10(-3) U/ml), the secretion of Tg was enhanced. The similar stimulation of cAMP was demonstrated in subcultures. These in vitro studies suggest that IL-1 beta may play a role in the pathogenesis of autoimmune thyroid diseases. Further, the stimulations at low concentrations indicate that IL-1 beta may regulate the function of the thyroid gland under physiological conditions.
AB - Cytokines are peptide hormones essential for cellular communication in the immune response. The purpose of this study was to investigate the influence of cytokines, especially recombinant interleukin 1 beta (rIL-1 beta), on human thyroid cells. Thyroglobulin (Tg) was measured by a double antibody radioimmunoassay, and cyclic AMP (cAMP) by a competitive protein binding assay. Supernatants from unstimulated and phytohaemagglutinin-stimulated blood mononuclear cells were added to human thyroid cells cultured in monolayers. A dose-dependent inhibition of the secretion of Tg and cAMP was demonstrated. Both subcultured and primary cultured cells incubated with rIL-1 beta at pharmacological levels (10(-1)-10(2) U/ml) exhibited an inhibition of Tg and cAMP secretion, while at physiological levels (10(-5)-10(-3) U/ml), the secretion of Tg was enhanced. The similar stimulation of cAMP was demonstrated in subcultures. These in vitro studies suggest that IL-1 beta may play a role in the pathogenesis of autoimmune thyroid diseases. Further, the stimulations at low concentrations indicate that IL-1 beta may regulate the function of the thyroid gland under physiological conditions.
KW - Autoimmune Diseases/metabolism
KW - Cells, Cultured
KW - Cyclic AMP/metabolism
KW - Humans
KW - Immunoenzyme Techniques
KW - Interleukin-1/pharmacology
KW - Thyroglobulin/metabolism
KW - Thyroid Gland/drug effects
KW - Thyrotropin/pharmacology
U2 - 10.1111/j.1398-9995.1988.tb00915.x
DO - 10.1111/j.1398-9995.1988.tb00915.x
M3 - Journal article
C2 - 2847577
SN - 0105-4538
VL - 43
SP - 435
EP - 441
JO - Allergy
JF - Allergy
IS - 6
ER -