Integrative Analysis of Fecal Metagenomics and Metabolomics in Colorectal Cancer

Marc Clos-Garcia; Koldo Garcia; Cristina Alonso; Marta Iruarrizaga-Lejarreta; Mauro D'Amato; Anais Crespo; Agueda Iglesias; Joaquín Cubiella; Luis Bujanda; Juan Manuel Falcón-Pérez

doi:10.3390/cancers12051142

Integrative Analysis of Fecal Metagenomics and Metabolomics in Colorectal Cancer

Marc Clos-Garcia, Koldo Garcia, Cristina Alonso, Marta Iruarrizaga-Lejarreta, Mauro D'Amato, Anais Crespo, Agueda Iglesias, Joaquín Cubiella, Luis Bujanda, Juan Manuel Falcón-Pérez

79 Citationer (Scopus)

Abstract

Although colorectal cancer (CRC) is the second leading cause of death in developed countries, current diagnostic tests for early disease stages are suboptimal. We have performed a combination of UHPLC-MS metabolomics and 16S microbiome analyses on 224 feces samples in order to identify early biomarkers for both advanced adenomas (AD) and CRC. We report differences in fecal levels of cholesteryl esters and sphingolipids in CRC. We identified Fusobacterium, Parvimonas and Staphylococcus to be increased in CRC patients and Lachnospiraceae family to be reduced. We finally described Adlercreutzia to be more abundant in AD patients' feces. Integration of metabolomics and microbiome data revealed tight interactions between bacteria and host and performed better than FOB test for CRC diagnosis. This study identifies potential early biomarkers that outperform current diagnostic tools and frame them into the stablished gut microbiota role in CRC pathogenesis.

Originalsprog	Engelsk
Tidsskrift	Cancers
Vol/bind	12
Udgave nummer	5
ISSN	2072-6694
DOI	https://doi.org/10.3390/cancers12051142
Status	Udgivet - 2 maj 2020
Udgivet eksternt	Ja

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10.3390/cancers12051142

Citationsformater

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title = "Integrative Analysis of Fecal Metagenomics and Metabolomics in Colorectal Cancer",

abstract = "Although colorectal cancer (CRC) is the second leading cause of death in developed countries, current diagnostic tests for early disease stages are suboptimal. We have performed a combination of UHPLC-MS metabolomics and 16S microbiome analyses on 224 feces samples in order to identify early biomarkers for both advanced adenomas (AD) and CRC. We report differences in fecal levels of cholesteryl esters and sphingolipids in CRC. We identified Fusobacterium, Parvimonas and Staphylococcus to be increased in CRC patients and Lachnospiraceae family to be reduced. We finally described Adlercreutzia to be more abundant in AD patients' feces. Integration of metabolomics and microbiome data revealed tight interactions between bacteria and host and performed better than FOB test for CRC diagnosis. This study identifies potential early biomarkers that outperform current diagnostic tools and frame them into the stablished gut microbiota role in CRC pathogenesis.",

author = "Marc Clos-Garcia and Koldo Garcia and Cristina Alonso and Marta Iruarrizaga-Lejarreta and Mauro D'Amato and Anais Crespo and Agueda Iglesias and Joaqu{\'i}n Cubiella and Luis Bujanda and Falc{\'o}n-P{\'e}rez, {Juan Manuel}",

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month = may,

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doi = "10.3390/cancers12051142",

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T1 - Integrative Analysis of Fecal Metagenomics and Metabolomics in Colorectal Cancer

AU - Clos-Garcia, Marc

AU - Garcia, Koldo

AU - Alonso, Cristina

AU - Iruarrizaga-Lejarreta, Marta

AU - D'Amato, Mauro

AU - Crespo, Anais

AU - Iglesias, Agueda

AU - Cubiella, Joaquín

AU - Bujanda, Luis

AU - Falcón-Pérez, Juan Manuel

PY - 2020/5/2

Y1 - 2020/5/2

N2 - Although colorectal cancer (CRC) is the second leading cause of death in developed countries, current diagnostic tests for early disease stages are suboptimal. We have performed a combination of UHPLC-MS metabolomics and 16S microbiome analyses on 224 feces samples in order to identify early biomarkers for both advanced adenomas (AD) and CRC. We report differences in fecal levels of cholesteryl esters and sphingolipids in CRC. We identified Fusobacterium, Parvimonas and Staphylococcus to be increased in CRC patients and Lachnospiraceae family to be reduced. We finally described Adlercreutzia to be more abundant in AD patients' feces. Integration of metabolomics and microbiome data revealed tight interactions between bacteria and host and performed better than FOB test for CRC diagnosis. This study identifies potential early biomarkers that outperform current diagnostic tools and frame them into the stablished gut microbiota role in CRC pathogenesis.

AB - Although colorectal cancer (CRC) is the second leading cause of death in developed countries, current diagnostic tests for early disease stages are suboptimal. We have performed a combination of UHPLC-MS metabolomics and 16S microbiome analyses on 224 feces samples in order to identify early biomarkers for both advanced adenomas (AD) and CRC. We report differences in fecal levels of cholesteryl esters and sphingolipids in CRC. We identified Fusobacterium, Parvimonas and Staphylococcus to be increased in CRC patients and Lachnospiraceae family to be reduced. We finally described Adlercreutzia to be more abundant in AD patients' feces. Integration of metabolomics and microbiome data revealed tight interactions between bacteria and host and performed better than FOB test for CRC diagnosis. This study identifies potential early biomarkers that outperform current diagnostic tools and frame them into the stablished gut microbiota role in CRC pathogenesis.

U2 - 10.3390/cancers12051142

DO - 10.3390/cancers12051142

M3 - Journal article

C2 - 32370168

SN - 2072-6694

VL - 12

JO - Cancers

JF - Cancers

IS - 5

ER -

Integrative Analysis of Fecal Metagenomics and Metabolomics in Colorectal Cancer

Abstract

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