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Insulin-like growth factor I enhances collagen synthesis in engineered human tendon tissue

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@article{1cfaba80b77e4acb8341ef483b22d6f4,
title = "Insulin-like growth factor I enhances collagen synthesis in engineered human tendon tissue",
abstract = "OBJECTIVE: Isolated human tendon cells form 3D tendon constructs that demonstrate collagen fibrillogenesis and feature structural similarities to tendon when cultured under tensile load. The exact role of circulating growth factors for collagen formation in tendon is sparsely examined. We investigated the influence of insulin-like growth factor I (IGF-I) on tendon construct formation in 3D cell culture.DESIGN: Tendon constructs were grown in 0.5 or 10% FBS with or without IGF-I (250 mg/ml) supplementation. Collagen content (fluorometric), mRNA levels (PCR) and fibril diameter (transmission electron microscopy) were determined at 7, 10, 14, 21 and 28 days.RESULTS: IGF-I revealed a stimulating effect on fibril diameter (up to day 21), mRNA for collagen (to day 28), tenomodulin (to day 28) and scleraxis (at days 10 and 14), and on overall collagen content. 10% FBS diminished the development of fibril diameter (day 14), collagen content (at days 21 and 28) and mRNA expression for collagen, tenomodulin and scleraxis.CONCLUSION: IGF-I supplementation promotes early onset of tensile load induced collagen formation and tendon structural arrangement, whereas the FBS concentration routinely used in cultures diminishes collagen expression, collagen content and fibril formation.",
keywords = "Adolescent, Adult, Collagen, Fibroblasts, Humans, In Vitro Techniques, Insulin-Like Growth Factor I, Microscopy, Electron, Transmission, RNA, Messenger, Reverse Transcriptase Polymerase Chain Reaction, Tendons, Tissue Engineering, Young Adult",
author = "Andreas Herchenhan and Bayer, {Monika L} and Pernilla Eliasson and Magnusson, {S Peter} and Michael Kjaer",
note = "Copyright {\textcopyright} 2014 Elsevier Ltd. All rights reserved.",
year = "2015",
month = feb,
doi = "10.1016/j.ghir.2014.09.001",
language = "English",
volume = "25",
pages = "13--9",
journal = "Growth Regulation",
issn = "1096-6374",
publisher = "Churchill Livingstone",
number = "1",

}

RIS

TY - JOUR

T1 - Insulin-like growth factor I enhances collagen synthesis in engineered human tendon tissue

AU - Herchenhan, Andreas

AU - Bayer, Monika L

AU - Eliasson, Pernilla

AU - Magnusson, S Peter

AU - Kjaer, Michael

N1 - Copyright © 2014 Elsevier Ltd. All rights reserved.

PY - 2015/2

Y1 - 2015/2

N2 - OBJECTIVE: Isolated human tendon cells form 3D tendon constructs that demonstrate collagen fibrillogenesis and feature structural similarities to tendon when cultured under tensile load. The exact role of circulating growth factors for collagen formation in tendon is sparsely examined. We investigated the influence of insulin-like growth factor I (IGF-I) on tendon construct formation in 3D cell culture.DESIGN: Tendon constructs were grown in 0.5 or 10% FBS with or without IGF-I (250 mg/ml) supplementation. Collagen content (fluorometric), mRNA levels (PCR) and fibril diameter (transmission electron microscopy) were determined at 7, 10, 14, 21 and 28 days.RESULTS: IGF-I revealed a stimulating effect on fibril diameter (up to day 21), mRNA for collagen (to day 28), tenomodulin (to day 28) and scleraxis (at days 10 and 14), and on overall collagen content. 10% FBS diminished the development of fibril diameter (day 14), collagen content (at days 21 and 28) and mRNA expression for collagen, tenomodulin and scleraxis.CONCLUSION: IGF-I supplementation promotes early onset of tensile load induced collagen formation and tendon structural arrangement, whereas the FBS concentration routinely used in cultures diminishes collagen expression, collagen content and fibril formation.

AB - OBJECTIVE: Isolated human tendon cells form 3D tendon constructs that demonstrate collagen fibrillogenesis and feature structural similarities to tendon when cultured under tensile load. The exact role of circulating growth factors for collagen formation in tendon is sparsely examined. We investigated the influence of insulin-like growth factor I (IGF-I) on tendon construct formation in 3D cell culture.DESIGN: Tendon constructs were grown in 0.5 or 10% FBS with or without IGF-I (250 mg/ml) supplementation. Collagen content (fluorometric), mRNA levels (PCR) and fibril diameter (transmission electron microscopy) were determined at 7, 10, 14, 21 and 28 days.RESULTS: IGF-I revealed a stimulating effect on fibril diameter (up to day 21), mRNA for collagen (to day 28), tenomodulin (to day 28) and scleraxis (at days 10 and 14), and on overall collagen content. 10% FBS diminished the development of fibril diameter (day 14), collagen content (at days 21 and 28) and mRNA expression for collagen, tenomodulin and scleraxis.CONCLUSION: IGF-I supplementation promotes early onset of tensile load induced collagen formation and tendon structural arrangement, whereas the FBS concentration routinely used in cultures diminishes collagen expression, collagen content and fibril formation.

KW - Adolescent

KW - Adult

KW - Collagen

KW - Fibroblasts

KW - Humans

KW - In Vitro Techniques

KW - Insulin-Like Growth Factor I

KW - Microscopy, Electron, Transmission

KW - RNA, Messenger

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Tendons

KW - Tissue Engineering

KW - Young Adult

U2 - 10.1016/j.ghir.2014.09.001

DO - 10.1016/j.ghir.2014.09.001

M3 - Journal article

C2 - 25281191

VL - 25

SP - 13

EP - 19

JO - Growth Regulation

JF - Growth Regulation

SN - 1096-6374

IS - 1

ER -

ID: 46033146