TY - JOUR
T1 - Insulin-like growth factor II mRNA, peptides, and receptors in a thoracopulmonary malignant small round cell tumor
AU - Nielsen, F C
AU - Orskov, C
AU - Haselbacher, G
AU - Ramlau, J
AU - Christiansen, J
AU - Schmiegelow, K
AU - Rehfeld, J F
PY - 1994/2/15
Y1 - 1994/2/15
N2 - Insulin-like growth factor-(IGF) II and IGF-I and IGF-II/mannose 6-phosphate receptors were expressed in a thoracopulmonary malignant small round cell tumor (MSRCT) from a 14-year-old boy. Northern analysis showed that the MSRCT expresses multiple IGF-II mRNA of 6.0, 4.8, 4.2, and 2.2 kilobase from promoters P3 and P4 of the human IGF-II gene. Chromatography and radioimmunoassay revealed two forms of IGF-II with molecular masses of 7.5 kilodalton (kDa) and 10 kDa, corresponding to mature IGF-II and IGF-II with a C-terminal extension, in concentrations of 61 and 41 ng/g/tumor tissue, respectively. By a combined reverse transcription-polymerase chain reaction analysis, the authors also show that the MSRCT expresses IGF-I and IGF-II/mannose 6-phosphate receptor mRNA. The plasma concentration of IGF-II was 600 ng/ml and within the normal range of serum IGF-II. IGF binding proteins (IGFBP) of 41.5, 38.5, 34, 30, and 24 kDa were present in serum. Compared with normal plasma from healthy subjects and an age-matched group of boys, the level of the 41.5, 38.5, and 30 kDa IGFBP were approximately 3-fold elevated. The authors conclude that transcription of the IGF-II gene leads to the production of significant amounts of 10 kDa IGF-II and 7.5 kDa IGF-II. IGF-II may stimulate the proliferation of MSRCT by interaction with IGF-I receptors on the cells.
AB - Insulin-like growth factor-(IGF) II and IGF-I and IGF-II/mannose 6-phosphate receptors were expressed in a thoracopulmonary malignant small round cell tumor (MSRCT) from a 14-year-old boy. Northern analysis showed that the MSRCT expresses multiple IGF-II mRNA of 6.0, 4.8, 4.2, and 2.2 kilobase from promoters P3 and P4 of the human IGF-II gene. Chromatography and radioimmunoassay revealed two forms of IGF-II with molecular masses of 7.5 kilodalton (kDa) and 10 kDa, corresponding to mature IGF-II and IGF-II with a C-terminal extension, in concentrations of 61 and 41 ng/g/tumor tissue, respectively. By a combined reverse transcription-polymerase chain reaction analysis, the authors also show that the MSRCT expresses IGF-I and IGF-II/mannose 6-phosphate receptor mRNA. The plasma concentration of IGF-II was 600 ng/ml and within the normal range of serum IGF-II. IGF binding proteins (IGFBP) of 41.5, 38.5, 34, 30, and 24 kDa were present in serum. Compared with normal plasma from healthy subjects and an age-matched group of boys, the level of the 41.5, 38.5, and 30 kDa IGFBP were approximately 3-fold elevated. The authors conclude that transcription of the IGF-II gene leads to the production of significant amounts of 10 kDa IGF-II and 7.5 kDa IGF-II. IGF-II may stimulate the proliferation of MSRCT by interaction with IGF-I receptors on the cells.
KW - Adolescent
KW - Blotting, Northern
KW - Carcinoma, Small Cell/metabolism
KW - Humans
KW - Immunoenzyme Techniques
KW - Insulin-Like Growth Factor I/biosynthesis
KW - Insulin-Like Growth Factor II/biosynthesis
KW - Lung Neoplasms/metabolism
KW - Male
KW - Polymerase Chain Reaction
KW - RNA, Messenger/analysis
KW - RNA, Neoplasm/analysis
KW - Receptor, IGF Type 1/analysis
KW - Receptor, IGF Type 2/analysis
KW - Thoracic Neoplasms/metabolism
U2 - 10.1002/1097-0142(19940215)73:4<1312::aid-cncr2820730429>3.0.co;2-d
DO - 10.1002/1097-0142(19940215)73:4<1312::aid-cncr2820730429>3.0.co;2-d
M3 - Journal article
C2 - 8313336
SN - 0008-543X
VL - 73
SP - 1312
EP - 1319
JO - Cancer
JF - Cancer
IS - 4
ER -