Influence of interleukin 6 on the function of secondary cultures of human thyrocytes

A Krogh Rasmussen, L Kayser, K Bech, U Feldt-Rasmussen, H Perrild, K Bendtzen

Abstract

Interleukin 6 has been suggested as second mediator of the effects of interleukin 1 in some cell systems. Interleukin 1 has previously been shown to inhibit the function of human thyrocytes in secondary cultures. We have therefore studied the influence of interleukin 6 (10(-1)-5.10(7) U/l) on the function of thyroid cells. Recombinant interleukin 6 slightly inhibited the production of cAMP, but failed to influence the production of thyroglobulin or the DNA content. Endotoxins (lipopolysaccharides from Salmonella abortus equi or Yersinia enterocolitica) had only a slightly inhibitory effect on thyroid cell functions, and the effect of interleukin 6 could not by itself be explained by endotoxin contamination. The effect of interleukin 6 did not mimic effects on thyroid cells afforded by recombinant interleukin 1 alpha and 1 beta. Furthermore, antibodies to interleukin 6 were not able to inhibit the interleukin 1 beta-induced inhibition of thyroid cell functions. In conclusion, it is unlikely that interleukin 6 by itself mediates the biological effects of interleukin 1 on human thyroid cells.

OriginalsprogEngelsk
TidsskriftActa Endocrinologica
Vol/bind124
Udgave nummer5
Sider (fra-til)577-82
Antal sider6
ISSN0001-5598
DOI
StatusUdgivet - maj 1991
Udgivet eksterntJa

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