Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

Increased susceptibility to diet-induced obesity in GPRC6A receptor knockout mice

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

DOI

  1. Hypophysectomy abolishes rhythms in rat thyroid hormones but not in the thyroid clock

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Interleukin-6 autoantibodies are involved in the pathogenesis of a subset of type 2 diabetes

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Expression and localization of the progesterone receptor in mouse and human reproductive organs

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Renal effects of a neutralising RAGE-antibody in long-term streptozotocin-diabetic mice

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Autoradiography as a Simple and Powerful Method for Visualization and Characterization of Pharmacological Targets

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Tryptophan depletion affects compulsive behaviour in rats: strain dependent effects and associated neuromechanisms

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Similar serotonin-2A receptor binding in rats with different coping styles or levels of aggression

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. A possible link between food and mood: dietary impact on gut microbiota and behavior in BALB/c mice

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  • Christoffer Clemmensen
  • Sanela Smajilovic
  • Andreas Nygaard Madsen
  • Anders B Klein
  • Birgitte Holst
  • Hans Bräuner-Osborne
Vis graf over relationer
The recently identified G protein-coupled receptor GPRC6A is activated by dietary amino acids and expressed in multiple tissues. Although the receptor is hypothesised to exert biological impact on metabolic and endocrine-related parameters, the role of the receptor in obesity and metabolic complications is still elusive. In the present study, we investigated the impact of GPRC6A deficiency in a murine model of diet-induced obesity (DIO). Male Gprc6a knockout (KO) mice and WT littermates were subjected to a high-fat diet (HFD) for 25 weeks and exposed to comprehensive metabolic phenotyping. A significant increase in body weight, corresponding to a selective increase in body fat, was observed in Gprc6a KO mice exposed to an HFD relative to WT controls. The obese phenotype was linked to subtle perturbations in energy homoeostasis as GPRC6A deficiency resulted in chronic hyperphagia and decreased locomotor activity. Moreover, diet-induced obese Gprc6a KO mice had increased circulating insulin and leptin levels relative to WT animals, thereby demonstrating that endocrine abnormalities associate with the reported disturbances in energy balance. The phenotype was further accompanied by disruptions in glucose metabolism showing that Gprc6a KO mice on an HFD display increased susceptibility to develop metabolic-related disorders. Altogether, these data suggest that the amino acid sensing receptor GPRC6A plays an important role in resistance to DIO and metabolic complications. Future studies will illuminate the underlying molecular mechanisms mediating the herein reported findings and potentially facilitate the development of novel therapeutic compounds targeting the GPRC6A receptor.
OriginalsprogEngelsk
TidsskriftJournal of Endocrinology
Vol/bind217
Udgave nummer2
Sider (fra-til)151-60
Antal sider10
ISSN0022-0795
DOI
StatusUdgivet - maj 2013

ID: 42576032