TY - JOUR
T1 - Increased intrafollicular androgen levels affect human granulosa cell secretion of anti-Müllerian hormone and inhibin-B
AU - Andersen, Claus Yding
AU - Lossl, Kristine
PY - 2008/6
Y1 - 2008/6
N2 - OBJECTIVE: To evaluate whether the follicular fluid concentrations of anti-müllerian hormone (AMH), inhibin-B, and P become affected by androgen priming before controlled ovarian hyperstimulation and IVF/intracytoplasmic sperm injection (ICSI) treatment, extending our previous study.DESIGN: Prospective nonrandomized clinical study.SETTING: University hospital.PATIENT(S): Patients (N = 45) undergoing IVF/ICSI treatment received androgen priming before controlled ovarian hyperstimulation.INTERVENTION(S): All patients received cetrorelix, 3 mg SC, on cycle day 2 and 5. Group I (n = 15) received no other pretreatment. Group II (n = 15) received 2.5 mg once daily of letrozole from cycle day 2 through 8. Group III (n = 15) received letrozole as group II plus 1,250 IU hCG on cycle day 2.MAIN OUTCOME MEASURE(S): Follicular fluid concentrations of AMH, inhibin-B, and steroids.RESULT(S): Follicular fluid concentrations of AMH were significantly higher in group III than in groups I and II (2.6 +/- 0.3 vs. 1.6 +/- 0.2 and 1.5 +/- 0.4 ng/mL. Concentrations of inhibin-B were significantly lower in group II and III as compared with group I (27 +/- 4, 27 +/- 3 versus 46 +/- 6 ng/mL, respectively). Follicular fluid P concentrations remained similar in all groups.CONCLUSION(S): Granulosa cell production of AMH was significantly augmented and inhibin-B production was significantly reduced by a highly specific pharmacologically induced increase in the intrafollicular androgen levels, suggesting a dual action of androgen on follicular growth and development.
AB - OBJECTIVE: To evaluate whether the follicular fluid concentrations of anti-müllerian hormone (AMH), inhibin-B, and P become affected by androgen priming before controlled ovarian hyperstimulation and IVF/intracytoplasmic sperm injection (ICSI) treatment, extending our previous study.DESIGN: Prospective nonrandomized clinical study.SETTING: University hospital.PATIENT(S): Patients (N = 45) undergoing IVF/ICSI treatment received androgen priming before controlled ovarian hyperstimulation.INTERVENTION(S): All patients received cetrorelix, 3 mg SC, on cycle day 2 and 5. Group I (n = 15) received no other pretreatment. Group II (n = 15) received 2.5 mg once daily of letrozole from cycle day 2 through 8. Group III (n = 15) received letrozole as group II plus 1,250 IU hCG on cycle day 2.MAIN OUTCOME MEASURE(S): Follicular fluid concentrations of AMH, inhibin-B, and steroids.RESULT(S): Follicular fluid concentrations of AMH were significantly higher in group III than in groups I and II (2.6 +/- 0.3 vs. 1.6 +/- 0.2 and 1.5 +/- 0.4 ng/mL. Concentrations of inhibin-B were significantly lower in group II and III as compared with group I (27 +/- 4, 27 +/- 3 versus 46 +/- 6 ng/mL, respectively). Follicular fluid P concentrations remained similar in all groups.CONCLUSION(S): Granulosa cell production of AMH was significantly augmented and inhibin-B production was significantly reduced by a highly specific pharmacologically induced increase in the intrafollicular androgen levels, suggesting a dual action of androgen on follicular growth and development.
KW - Androgens/metabolism
KW - Androsterone/metabolism
KW - Anti-Mullerian Hormone/metabolism
KW - Embryo Implantation
KW - Embryo Transfer
KW - Estradiol/metabolism
KW - Female
KW - Fertilization in Vitro
KW - Follicular Fluid/chemistry
KW - Gonadotropin-Releasing Hormone/analogs & derivatives
KW - Granulosa Cells/metabolism
KW - Hormone Antagonists/therapeutic use
KW - Humans
KW - Inhibins/metabolism
KW - Ovarian Follicle/physiology
KW - Ovulation Induction
KW - Progesterone/metabolism
KW - Prospective Studies
KW - Sperm Injections, Intracytoplasmic
KW - Testosterone/metabolism
U2 - 10.1016/j.fertnstert.2007.05.003
DO - 10.1016/j.fertnstert.2007.05.003
M3 - Journal article
C2 - 17628551
SN - 0015-0282
VL - 89
SP - 1760
EP - 1765
JO - Fertility and Sterility
JF - Fertility and Sterility
IS - 6
ER -