TY - JOUR
T1 - Increased cardiac BNP expression associated with myocardial ischemia
AU - Goetze, J P
AU - Christoffersen, C
AU - Perko, M
AU - Arendrup, H
AU - Rehfeld, J F
AU - Kastrup, J
AU - Nielsen, L B
PY - 2003/6
Y1 - 2003/6
N2 - Congestive heart failure is accompanied by increased cardiac brain natriuretic peptide (BNP) gene expression with elevated plasma concentrations of BNP and its precursor, proBNP. We investigated if myocardial ischemia in the absence of overt heart failure may be another mechanism for increased myocardial BNP expression. The BNP expression was examined in hypoxic myocardium of patients undergoing coronary bypass grafting surgery, in patients with coronary artery disease and normal left ventricular function undergoing percutaneous transluminal intervention therapy, and in heart failure patients without coronary artery disease. BNP mRNA was quantified by real-time PCR, and plasma BNP and proBNP concentrations were measured with radioimmunoassays. Quantitative analysis of BNP mRNA in atrial and ventricular biopsies from coronary bypass grafting patients revealed close associations of plasma BNP and proBNP concentrations to ventricular, but not atrial, BNP mRNA levels. Plasma BNP and proBNP concentrations were markedly increased in patients with coronary artery disease but without concomitant left ventricular dysfunction. These results are compatible with the notion that myocardial ischemia, even in the absence of left ventricular dysfunction, augments cardiac BNP gene expression and increases plasma BNP and proBNP concentrations. Thus, elevated BNP and proBNP concentrations do not necessarily reflect heart failure but may also result from cardiac ischemia.
AB - Congestive heart failure is accompanied by increased cardiac brain natriuretic peptide (BNP) gene expression with elevated plasma concentrations of BNP and its precursor, proBNP. We investigated if myocardial ischemia in the absence of overt heart failure may be another mechanism for increased myocardial BNP expression. The BNP expression was examined in hypoxic myocardium of patients undergoing coronary bypass grafting surgery, in patients with coronary artery disease and normal left ventricular function undergoing percutaneous transluminal intervention therapy, and in heart failure patients without coronary artery disease. BNP mRNA was quantified by real-time PCR, and plasma BNP and proBNP concentrations were measured with radioimmunoassays. Quantitative analysis of BNP mRNA in atrial and ventricular biopsies from coronary bypass grafting patients revealed close associations of plasma BNP and proBNP concentrations to ventricular, but not atrial, BNP mRNA levels. Plasma BNP and proBNP concentrations were markedly increased in patients with coronary artery disease but without concomitant left ventricular dysfunction. These results are compatible with the notion that myocardial ischemia, even in the absence of left ventricular dysfunction, augments cardiac BNP gene expression and increases plasma BNP and proBNP concentrations. Thus, elevated BNP and proBNP concentrations do not necessarily reflect heart failure but may also result from cardiac ischemia.
KW - Angioplasty, Balloon, Coronary
KW - Coronary Artery Bypass
KW - Coronary Artery Disease/metabolism
KW - Gene Expression Regulation
KW - Heart Atria/metabolism
KW - Heart Ventricles/metabolism
KW - Humans
KW - Models, Cardiovascular
KW - Myocardial Ischemia/genetics
KW - Myocardium/metabolism
KW - Natriuretic Peptide, Brain/biosynthesis
KW - Nerve Tissue Proteins/blood
KW - Peptide Fragments/blood
KW - RNA, Messenger/analysis
U2 - 10.1096/fj.02-0796fje
DO - 10.1096/fj.02-0796fje
M3 - Journal article
C2 - 12709407
SN - 0892-6638
VL - 17
SP - 1105
EP - 1107
JO - FASEB Journal
JF - FASEB Journal
IS - 9
ER -