In Vivo Selection of a Unique Tandem Repeat Mediated Azole Resistance Mechanism (TR120) in Aspergillus fumigatus cyp51A, Denmark

Rasmus K Hare; Jan B Gertsen; Karen M T Astvad; Kristine B Degn; Anders Løkke; Marc Stegger; Paal S Andersen; Lise Kristensen; Maiken C Arendrup

doi:10.3201/eid2503.180297

In Vivo Selection of a Unique Tandem Repeat Mediated Azole Resistance Mechanism (TR120) in Aspergillus fumigatus cyp51A, Denmark

Rasmus K Hare, Jan B Gertsen, Karen M T Astvad, Kristine B Degn, Anders Løkke, Marc Stegger, Paal S Andersen, Lise Kristensen, Maiken C Arendrup

47 Citationer (Scopus)

Abstract

We report a fatal aspergillosis case in which STRAf typing and whole-genome sequencing substantiated in vivo emergence of an azole-resistant Aspergillus fumigatus with a 120-bp tandem repeat in the promoter region of cyp51A. This event, previously restricted to the environment, challenges current understanding of azole resistance development in A. fumigatus.

Originalsprog	Engelsk
Tidsskrift	Emerging Infectious Diseases (Print Edition)
Vol/bind	25
Udgave nummer	3
Sider (fra-til)	577-580
Antal sider	4
ISSN	1080-6040
DOI	https://doi.org/10.3201/eid2503.180297
Status	Udgivet - mar. 2019

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Hare, R. K., Gertsen, J. B., Astvad, K. M. T., Degn, K. B., Løkke, A., Stegger, M., Andersen, P. S., Kristensen, L., & Arendrup, M. C. (2019). In Vivo Selection of a Unique Tandem Repeat Mediated Azole Resistance Mechanism (TR120) in Aspergillus fumigatus cyp51A, Denmark. Emerging Infectious Diseases (Print Edition), 25(3), 577-580. https://doi.org/10.3201/eid2503.180297

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abstract = "We report a fatal aspergillosis case in which STRAf typing and whole-genome sequencing substantiated in vivo emergence of an azole-resistant Aspergillus fumigatus with a 120-bp tandem repeat in the promoter region of cyp51A. This event, previously restricted to the environment, challenges current understanding of azole resistance development in A. fumigatus.",

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AU - Astvad, Karen M T

AU - Degn, Kristine B

AU - Løkke, Anders

AU - Stegger, Marc

AU - Andersen, Paal S

AU - Kristensen, Lise

AU - Arendrup, Maiken C

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AB - We report a fatal aspergillosis case in which STRAf typing and whole-genome sequencing substantiated in vivo emergence of an azole-resistant Aspergillus fumigatus with a 120-bp tandem repeat in the promoter region of cyp51A. This event, previously restricted to the environment, challenges current understanding of azole resistance development in A. fumigatus.

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KW - Aspergillus fumigatus/drug effects

KW - Azoles/pharmacology

KW - Cytochrome P-450 Enzyme System/genetics

KW - Denmark/epidemiology

KW - Drug Resistance, Fungal

KW - Fungal Proteins/genetics

KW - Humans

KW - Male

KW - Middle Aged

KW - Mutation

KW - Phylogeny

KW - Promoter Regions, Genetic

KW - Selection, Genetic

KW - Tandem Repeat Sequences

KW - Tomography, X-Ray Computed

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In Vivo Selection of a Unique Tandem Repeat Mediated Azole Resistance Mechanism (TR120) in Aspergillus fumigatus cyp51A, Denmark

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