Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

In vivo experimental stroke and in vitro organ culture induce similar changes in vasoconstrictor receptors and intracellular calcium handling in rat cerebral arteries

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

DOI

  1. Sensory defunctionalization induced by 8% topical capsaicin treatment in a model of ultraviolet-B-induced cutaneous hyperalgesia

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Influence of visual feedback, hand dominance and sex on individuated finger movements

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Improved assessment of outcomes following transient global cerebral ischemia in mice

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Effects of dopaminergic treatment on functional cortico-cortical connectivity in Parkinson's disease

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  5. Cue integration and the perception of action in intentional binding

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Hormonal influences in migraine - interactions of oestrogen, oxytocin and CGRP

    Publikation: Bidrag til tidsskriftReviewForskningpeer review

  2. CGRP and migraine; from bench to bedside

    Publikation: Bidrag til tidsskriftReviewForskningpeer review

  3. Pre-Chiasmatic, Single Injection of Autologous Blood to Induce Experimental Subarachnoid Hemorrhage in a Rat Model

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Vis graf over relationer
Cerebral arteries subjected to different types of experimental stroke upregulate their expression of certain G-protein-coupled vasoconstrictor receptors, a phenomenon that worsens the ischemic brain damage. Upregulation of contractile endothelin B (ET(B)) and 5-hydroxytryptamine 1B (5-HT(1B)) receptors has been demonstrated after subarachnoid hemorrhage and global ischemic stroke, but the situation is less clear after focal ischemic stroke. Changes in smooth muscle calcium handling have been implicated in different vascular diseases but have not hitherto been investigated in cerebral arteries after stroke. Here, we evaluate changes of ET(B) and 5-HT(1B) receptors, intracellular calcium levels, and calcium channel expression in rat middle cerebral artery (MCA) after focal cerebral ischemia and in vitro organ culture, a proposed model of vasoconstrictor receptor changes after stroke. Rats were subjected to 2 h MCA occlusion followed by reperfusion for 1 or 24 h. Alternatively, MCAs from naïve rats were cultured for 1 or 24 h. ET(B) and 5-HT(1B) receptor-mediated contractions were evaluated by wire myography. Receptor and channel expressions were measured by real-time PCR and immunohistochemistry. Intracellular calcium was measured by FURA-2. Expression and contractile functions of ET(B) and 5-HT(1B) receptors were strongly upregulated and slightly downregulated, respectively, 24 h after experimental stroke or organ culture. ET(B) receptor-mediated contraction was mediated by calcium from intracellular and extracellular sources, whereas 5-HT(1B) receptor-mediated contraction was solely dependent on extracellular calcium. Organ culture and stroke increased basal intracellular calcium levels in MCA smooth muscle cells and decreased the expression of inositol triphosphate receptor and transient receptor potential canonical calcium channels, but not voltage-operated calcium channels.
OriginalsprogEngelsk
TidsskriftExperimental Brain Research
Vol/bind219
Udgave nummer4
Sider (fra-til)507-20
Antal sider14
ISSN0014-4819
DOI
StatusUdgivet - 2012

ID: 36832856