In vitro and in vivo effects of polyethylene glycol (PEG)-modified lipid in DOTAP/cholesterol-mediated gene transfection

Torben Gjetting; Nicolai Skovbjerg Arildsen; Camilla Laulund Christensen; Thomas Tuxen Poulsen; Jack A Roth; Vagn Neerup Handlos; Hans Skovgaard Poulsen

doi:10.2147/IJN.S10462

In vitro and in vivo effects of polyethylene glycol (PEG)-modified lipid in DOTAP/cholesterol-mediated gene transfection

Torben Gjetting, Nicolai Skovbjerg Arildsen, Camilla Laulund Christensen, Thomas Tuxen Poulsen, Jack A Roth, Vagn Neerup Handlos, Hans Skovgaard Poulsen

97 Citationer (Scopus)

Abstract

DOTAP/cholesterol-based lipoplexes are successfully used for delivery of plasmid DNA in vivo especially to the lungs, although low systemic stability and circulation have been reported. To achieve the aim of discovering the best method for systemic delivery of DNA to disseminated tumors we evaluated the potential of formulating DOTAP/cholesterol lipoplexes with a polyethylene glycol (PEG)-modified lipid, giving the benefit of the shielding and stabilizing properties of PEG in the bloodstream.

Originalsprog	Engelsk
Tidsskrift	International Journal of Nanomedicine
Vol/bind	5
Sider (fra-til)	371-83
Antal sider	13
ISSN	1176-9114
DOI	https://doi.org/10.2147/IJN.S10462
Status	Udgivet - 9 aug. 2010

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title = "In vitro and in vivo effects of polyethylene glycol (PEG)-modified lipid in DOTAP/cholesterol-mediated gene transfection",

abstract = "DOTAP/cholesterol-based lipoplexes are successfully used for delivery of plasmid DNA in vivo especially to the lungs, although low systemic stability and circulation have been reported. To achieve the aim of discovering the best method for systemic delivery of DNA to disseminated tumors we evaluated the potential of formulating DOTAP/cholesterol lipoplexes with a polyethylene glycol (PEG)-modified lipid, giving the benefit of the shielding and stabilizing properties of PEG in the bloodstream.",

author = "Torben Gjetting and Arildsen, \{Nicolai Skovbjerg\} and Christensen, \{Camilla Laulund\} and Poulsen, \{Thomas Tuxen\} and Roth, \{Jack A\} and Handlos, \{Vagn Neerup\} and Poulsen, \{Hans Skovgaard\}",

year = "2010",

month = aug,

day = "9",

doi = "10.2147/IJN.S10462",

language = "English",

volume = "5",

pages = "371--83",

journal = "International Journal of Nanomedicine",

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TY - JOUR

T1 - In vitro and in vivo effects of polyethylene glycol (PEG)-modified lipid in DOTAP/cholesterol-mediated gene transfection

AU - Gjetting, Torben

AU - Arildsen, Nicolai Skovbjerg

AU - Christensen, Camilla Laulund

AU - Poulsen, Thomas Tuxen

AU - Roth, Jack A

AU - Handlos, Vagn Neerup

AU - Poulsen, Hans Skovgaard

PY - 2010/8/9

Y1 - 2010/8/9

N2 - DOTAP/cholesterol-based lipoplexes are successfully used for delivery of plasmid DNA in vivo especially to the lungs, although low systemic stability and circulation have been reported. To achieve the aim of discovering the best method for systemic delivery of DNA to disseminated tumors we evaluated the potential of formulating DOTAP/cholesterol lipoplexes with a polyethylene glycol (PEG)-modified lipid, giving the benefit of the shielding and stabilizing properties of PEG in the bloodstream.

AB - DOTAP/cholesterol-based lipoplexes are successfully used for delivery of plasmid DNA in vivo especially to the lungs, although low systemic stability and circulation have been reported. To achieve the aim of discovering the best method for systemic delivery of DNA to disseminated tumors we evaluated the potential of formulating DOTAP/cholesterol lipoplexes with a polyethylene glycol (PEG)-modified lipid, giving the benefit of the shielding and stabilizing properties of PEG in the bloodstream.

UR - https://www.scopus.com/pages/publications/79952118598

U2 - 10.2147/IJN.S10462

DO - 10.2147/IJN.S10462

M3 - Journal article

C2 - 20957159

SN - 1176-9114

VL - 5

SP - 371

EP - 383

JO - International Journal of Nanomedicine

JF - International Journal of Nanomedicine

ER -

In vitro and in vivo effects of polyethylene glycol (PEG)-modified lipid in DOTAP/cholesterol-mediated gene transfection

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