TY - JOUR
T1 - In vitro and in vivo effects of polyethylene glycol (PEG)-modified lipid in DOTAP/cholesterol-mediated gene transfection
AU - Gjetting, Torben
AU - Arildsen, Nicolai Skovbjerg
AU - Christensen, Camilla Laulund
AU - Poulsen, Thomas Tuxen
AU - Roth, Jack A
AU - Handlos, Vagn Neerup
AU - Poulsen, Hans Skovgaard
PY - 2010/8/9
Y1 - 2010/8/9
N2 - DOTAP/cholesterol-based lipoplexes are successfully used for delivery of plasmid DNA in vivo especially to the lungs, although low systemic stability and circulation have been reported. To achieve the aim of discovering the best method for systemic delivery of DNA to disseminated tumors we evaluated the potential of formulating DOTAP/cholesterol lipoplexes with a polyethylene glycol (PEG)-modified lipid, giving the benefit of the shielding and stabilizing properties of PEG in the bloodstream.
AB - DOTAP/cholesterol-based lipoplexes are successfully used for delivery of plasmid DNA in vivo especially to the lungs, although low systemic stability and circulation have been reported. To achieve the aim of discovering the best method for systemic delivery of DNA to disseminated tumors we evaluated the potential of formulating DOTAP/cholesterol lipoplexes with a polyethylene glycol (PEG)-modified lipid, giving the benefit of the shielding and stabilizing properties of PEG in the bloodstream.
U2 - 10.2147/IJN.S10462
DO - 10.2147/IJN.S10462
M3 - Journal article
C2 - 20957159
SN - 1176-9114
VL - 5
SP - 371
EP - 383
JO - International Journal of Nanomedicine
JF - International Journal of Nanomedicine
ER -