Improvement in fetal DNA extraction from maternal plasma. Evaluation of the NucliSens Magnetic Extraction system and the QIAamp DSP Virus Kit in comparison with the QIAamp DNA Blood Mini Kit

Frederik Banch Clausen; Grethe Risum Krog; Klaus Rieneck; Morten Hanefeld Dziegiel

doi:10.1002/pd.1605

Improvement in fetal DNA extraction from maternal plasma. Evaluation of the NucliSens Magnetic Extraction system and the QIAamp DSP Virus Kit in comparison with the QIAamp DNA Blood Mini Kit

Frederik Banch Clausen, Grethe Risum Krog, Klaus Rieneck, Morten Hanefeld Dziegiel

Afdeling for Klinisk Immunologi DIA

55 Citationer (Scopus)

Abstract

OBJECTIVE: Prenatal diagnostic assays have been developed using free fetal DNA circulating in the maternal blood of pregnant women. Efficient DNA extraction is crucial for a robust analysis. To improve fetal DNA yield, we tested two manual extraction methods--the NucliSens Magnetic Extraction (NMAG) system and the QIAamp DSP Virus Kit (QDSP)--against our current standard method, the widely used QIAamp DNA Blood Mini Kit (QDNA).

METHODS: The fetal DNA yield of the two extraction systems was evaluated using the RHD exon 7 as target in DNA extracts of 75 plasma samples from pregnant RhD-negative women, known to have given birth to RhD-positive infanto. The total DNA yield was evaluated in 23 samples, targeting GAPDH.

RESULTS: The fetal DNA yield was improved by a mean factor of 1.7 using the NMAG system, and improved by a mean factor of 1.5 using the QDSP. The total DNA yield was improved by a mean factor of 2.3 using the NMAG system, and by a mean factor of 1.3 using the QDSP.

CONCLUSION: Both extraction systems tested were superior to our standard with regard to DNA yield. This improvement may have a great impact on the success of genotyping in early pregnancy.

Originalsprog	Engelsk
Tidsskrift	Prenatal Diagnosis
Vol/bind	27
Udgave nummer	1
Sider (fra-til)	6-10
Antal sider	5
ISSN	0197-3851
DOI	https://doi.org/10.1002/pd.1605
Status	Udgivet - jan. 2007

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10.1002/pd.1605

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Improvement in fetal DNA extraction from maternal plasma. Evaluation of the NucliSens Magnetic Extraction system and the QIAamp DSP Virus Kit in comparison with the QIAamp DNA Blood Mini Kit. / Clausen, Frederik Banch ; Krog, Grethe Risum ; Rieneck, Klaus et al.
I: Prenatal Diagnosis, Bind 27, Nr. 1, 01.2007, s. 6-10.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

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title = "Improvement in fetal DNA extraction from maternal plasma. Evaluation of the NucliSens Magnetic Extraction system and the QIAamp DSP Virus Kit in comparison with the QIAamp DNA Blood Mini Kit",

abstract = "OBJECTIVE: Prenatal diagnostic assays have been developed using free fetal DNA circulating in the maternal blood of pregnant women. Efficient DNA extraction is crucial for a robust analysis. To improve fetal DNA yield, we tested two manual extraction methods--the NucliSens Magnetic Extraction (NMAG) system and the QIAamp DSP Virus Kit (QDSP)--against our current standard method, the widely used QIAamp DNA Blood Mini Kit (QDNA).METHODS: The fetal DNA yield of the two extraction systems was evaluated using the RHD exon 7 as target in DNA extracts of 75 plasma samples from pregnant RhD-negative women, known to have given birth to RhD-positive infanto. The total DNA yield was evaluated in 23 samples, targeting GAPDH.RESULTS: The fetal DNA yield was improved by a mean factor of 1.7 using the NMAG system, and improved by a mean factor of 1.5 using the QDSP. The total DNA yield was improved by a mean factor of 2.3 using the NMAG system, and by a mean factor of 1.3 using the QDSP.CONCLUSION: Both extraction systems tested were superior to our standard with regard to DNA yield. This improvement may have a great impact on the success of genotyping in early pregnancy.",

keywords = "DNA/blood, Exons, Female, Fetal Blood/chemistry, Fetomaternal Transfusion/blood, Glyceraldehyde-3-Phosphate Dehydrogenases/genetics, Humans, Magnetics, Polymerase Chain Reaction/methods, Pregnancy, Prenatal Diagnosis/methods, Reagent Kits, Diagnostic, Rh-Hr Blood-Group System/genetics",

author = "Clausen, {Frederik Banch} and Krog, {Grethe Risum} and Klaus Rieneck and Dziegiel, {Morten Hanefeld}",

year = "2007",

month = jan,

doi = "10.1002/pd.1605",

language = "English",

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T1 - Improvement in fetal DNA extraction from maternal plasma. Evaluation of the NucliSens Magnetic Extraction system and the QIAamp DSP Virus Kit in comparison with the QIAamp DNA Blood Mini Kit

AU - Clausen, Frederik Banch

AU - Krog, Grethe Risum

AU - Rieneck, Klaus

AU - Dziegiel, Morten Hanefeld

PY - 2007/1

Y1 - 2007/1

N2 - OBJECTIVE: Prenatal diagnostic assays have been developed using free fetal DNA circulating in the maternal blood of pregnant women. Efficient DNA extraction is crucial for a robust analysis. To improve fetal DNA yield, we tested two manual extraction methods--the NucliSens Magnetic Extraction (NMAG) system and the QIAamp DSP Virus Kit (QDSP)--against our current standard method, the widely used QIAamp DNA Blood Mini Kit (QDNA).METHODS: The fetal DNA yield of the two extraction systems was evaluated using the RHD exon 7 as target in DNA extracts of 75 plasma samples from pregnant RhD-negative women, known to have given birth to RhD-positive infanto. The total DNA yield was evaluated in 23 samples, targeting GAPDH.RESULTS: The fetal DNA yield was improved by a mean factor of 1.7 using the NMAG system, and improved by a mean factor of 1.5 using the QDSP. The total DNA yield was improved by a mean factor of 2.3 using the NMAG system, and by a mean factor of 1.3 using the QDSP.CONCLUSION: Both extraction systems tested were superior to our standard with regard to DNA yield. This improvement may have a great impact on the success of genotyping in early pregnancy.

AB - OBJECTIVE: Prenatal diagnostic assays have been developed using free fetal DNA circulating in the maternal blood of pregnant women. Efficient DNA extraction is crucial for a robust analysis. To improve fetal DNA yield, we tested two manual extraction methods--the NucliSens Magnetic Extraction (NMAG) system and the QIAamp DSP Virus Kit (QDSP)--against our current standard method, the widely used QIAamp DNA Blood Mini Kit (QDNA).METHODS: The fetal DNA yield of the two extraction systems was evaluated using the RHD exon 7 as target in DNA extracts of 75 plasma samples from pregnant RhD-negative women, known to have given birth to RhD-positive infanto. The total DNA yield was evaluated in 23 samples, targeting GAPDH.RESULTS: The fetal DNA yield was improved by a mean factor of 1.7 using the NMAG system, and improved by a mean factor of 1.5 using the QDSP. The total DNA yield was improved by a mean factor of 2.3 using the NMAG system, and by a mean factor of 1.3 using the QDSP.CONCLUSION: Both extraction systems tested were superior to our standard with regard to DNA yield. This improvement may have a great impact on the success of genotyping in early pregnancy.

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KW - Exons

KW - Female

KW - Fetal Blood/chemistry

KW - Fetomaternal Transfusion/blood

KW - Glyceraldehyde-3-Phosphate Dehydrogenases/genetics

KW - Humans

KW - Magnetics

KW - Polymerase Chain Reaction/methods

KW - Pregnancy

KW - Prenatal Diagnosis/methods

KW - Reagent Kits, Diagnostic

KW - Rh-Hr Blood-Group System/genetics

U2 - 10.1002/pd.1605

DO - 10.1002/pd.1605

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ER -

Improvement in fetal DNA extraction from maternal plasma. Evaluation of the NucliSens Magnetic Extraction system and the QIAamp DSP Virus Kit in comparison with the QIAamp DNA Blood Mini Kit

Abstract

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