Improved synthesis methods of standards used for quantitative determination of total isothiocyanates from broccoli in human urine

Mette Kristensen, Kirstine S Krogholm, Hanne Frederiksen, Fritz Duus, Claus Cornett, Susanne H Bügel, Salka E Rasmussen

12 Citationer (Scopus)

Abstract

A well-known method for quantification of isothiocyanates (ITCs) and their metabolites is the condensation reaction with 1,2-benzenedithiole to produce 1,3-benzodithiole-2-thione, which can be quantified by high-performance liquid chromatography. Standards of an ITC metabolite and 1,3-benzodithiole-2-thione are required for this assay but are not commercially available. In the present study, we report on an improved synthesis of the ITC metabolite N-acetyl-S-(N-4-methylsulfinylbutylthiocarbamoyl)-L-cysteine and 1,3-benzodithiole-2-thione. The standards were used to quantify the urinary excretion of ITCs from 10 healthy subjects who consumed 350 g broccoli. The excretion was investigated throughout 48 h showing a cumulative urinary ITC excretion of 49.1+/-25.2% of the dose.

OriginalsprogEngelsk
TidsskriftJournal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Vol/bind852
Udgave nummer1-2
Sider (fra-til)229-34
Antal sider6
ISSN1570-0232
DOI
StatusUdgivet - 1 jun. 2007

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