Improved synthesis methods of standards used for quantitative determination of total isothiocyanates from broccoli in human urine

Mette Kristensen; Kirstine S Krogholm; Hanne Frederiksen; Fritz Duus; Claus Cornett; Susanne H Bügel; Salka E Rasmussen

doi:10.1016/j.jchromb.2007.01.022

Improved synthesis methods of standards used for quantitative determination of total isothiocyanates from broccoli in human urine

Mette Kristensen, Kirstine S Krogholm, Hanne Frederiksen, Fritz Duus, Claus Cornett, Susanne H Bügel, Salka E Rasmussen

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11 Citationer (Scopus)

Abstrakt

A well-known method for quantification of isothiocyanates (ITCs) and their metabolites is the condensation reaction with 1,2-benzenedithiole to produce 1,3-benzodithiole-2-thione, which can be quantified by high-performance liquid chromatography. Standards of an ITC metabolite and 1,3-benzodithiole-2-thione are required for this assay but are not commercially available. In the present study, we report on an improved synthesis of the ITC metabolite N-acetyl-S-(N-4-methylsulfinylbutylthiocarbamoyl)-L-cysteine and 1,3-benzodithiole-2-thione. The standards were used to quantify the urinary excretion of ITCs from 10 healthy subjects who consumed 350 g broccoli. The excretion was investigated throughout 48 h showing a cumulative urinary ITC excretion of 49.1+/-25.2% of the dose.

Originalsprog	Engelsk
Tidsskrift	Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Vol/bind	852
Udgave nummer	1-2
Sider (fra-til)	229-34
Antal sider	6
ISSN	1570-0232
DOI	https://doi.org/10.1016/j.jchromb.2007.01.022
Status	Udgivet - 1 jun. 2007

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10.1016/j.jchromb.2007.01.022

Citationsformater

Kristensen, M., Krogholm, K. S., Frederiksen, H., Duus, F., Cornett, C., Bügel, S. H., & Rasmussen, S. E. (2007). Improved synthesis methods of standards used for quantitative determination of total isothiocyanates from broccoli in human urine. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 852(1-2), 229-34. https://doi.org/10.1016/j.jchromb.2007.01.022

Improved synthesis methods of standards used for quantitative determination of total isothiocyanates from broccoli in human urine. / Kristensen, Mette; Krogholm, Kirstine S; Frederiksen, Hanne et al.

I: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, Bind 852, Nr. 1-2, 01.06.2007, s. 229-34.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Kristensen, M, Krogholm, KS, Frederiksen, H, Duus, F, Cornett, C, Bügel, SH & Rasmussen, SE 2007, 'Improved synthesis methods of standards used for quantitative determination of total isothiocyanates from broccoli in human urine', Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, bind 852, nr. 1-2, s. 229-34. https://doi.org/10.1016/j.jchromb.2007.01.022

Kristensen M, Krogholm KS, Frederiksen H, Duus F, Cornett C, Bügel SH et al. Improved synthesis methods of standards used for quantitative determination of total isothiocyanates from broccoli in human urine. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences. 2007 jun. 1;852(1-2):229-34. https://doi.org/10.1016/j.jchromb.2007.01.022

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title = "Improved synthesis methods of standards used for quantitative determination of total isothiocyanates from broccoli in human urine",

abstract = "A well-known method for quantification of isothiocyanates (ITCs) and their metabolites is the condensation reaction with 1,2-benzenedithiole to produce 1,3-benzodithiole-2-thione, which can be quantified by high-performance liquid chromatography. Standards of an ITC metabolite and 1,3-benzodithiole-2-thione are required for this assay but are not commercially available. In the present study, we report on an improved synthesis of the ITC metabolite N-acetyl-S-(N-4-methylsulfinylbutylthiocarbamoyl)-L-cysteine and 1,3-benzodithiole-2-thione. The standards were used to quantify the urinary excretion of ITCs from 10 healthy subjects who consumed 350 g broccoli. The excretion was investigated throughout 48 h showing a cumulative urinary ITC excretion of 49.1+/-25.2% of the dose.",

keywords = "Adult, Brassica, Calibration, Chromatography, High Pressure Liquid, Female, Humans, Isothiocyanates, Male, Reference Standards, Spectrophotometry, Ultraviolet",

author = "Mette Kristensen and Krogholm, {Kirstine S} and Hanne Frederiksen and Fritz Duus and Claus Cornett and B{\"u}gel, {Susanne H} and Rasmussen, {Salka E}",

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T1 - Improved synthesis methods of standards used for quantitative determination of total isothiocyanates from broccoli in human urine

AU - Kristensen, Mette

AU - Krogholm, Kirstine S

AU - Frederiksen, Hanne

AU - Duus, Fritz

AU - Cornett, Claus

AU - Bügel, Susanne H

AU - Rasmussen, Salka E

PY - 2007/6/1

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N2 - A well-known method for quantification of isothiocyanates (ITCs) and their metabolites is the condensation reaction with 1,2-benzenedithiole to produce 1,3-benzodithiole-2-thione, which can be quantified by high-performance liquid chromatography. Standards of an ITC metabolite and 1,3-benzodithiole-2-thione are required for this assay but are not commercially available. In the present study, we report on an improved synthesis of the ITC metabolite N-acetyl-S-(N-4-methylsulfinylbutylthiocarbamoyl)-L-cysteine and 1,3-benzodithiole-2-thione. The standards were used to quantify the urinary excretion of ITCs from 10 healthy subjects who consumed 350 g broccoli. The excretion was investigated throughout 48 h showing a cumulative urinary ITC excretion of 49.1+/-25.2% of the dose.

AB - A well-known method for quantification of isothiocyanates (ITCs) and their metabolites is the condensation reaction with 1,2-benzenedithiole to produce 1,3-benzodithiole-2-thione, which can be quantified by high-performance liquid chromatography. Standards of an ITC metabolite and 1,3-benzodithiole-2-thione are required for this assay but are not commercially available. In the present study, we report on an improved synthesis of the ITC metabolite N-acetyl-S-(N-4-methylsulfinylbutylthiocarbamoyl)-L-cysteine and 1,3-benzodithiole-2-thione. The standards were used to quantify the urinary excretion of ITCs from 10 healthy subjects who consumed 350 g broccoli. The excretion was investigated throughout 48 h showing a cumulative urinary ITC excretion of 49.1+/-25.2% of the dose.

KW - Adult

KW - Brassica

KW - Calibration

KW - Chromatography, High Pressure Liquid

KW - Female

KW - Humans

KW - Isothiocyanates

KW - Male

KW - Reference Standards

KW - Spectrophotometry, Ultraviolet

U2 - 10.1016/j.jchromb.2007.01.022

DO - 10.1016/j.jchromb.2007.01.022

M3 - Journal article

C2 - 17307405

VL - 852

SP - 229

EP - 234

JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

SN - 1570-0232

IS - 1-2

ER -

Improved synthesis methods of standards used for quantitative determination of total isothiocyanates from broccoli in human urine

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