Impaired alpha-carboxyamidation of gastrin in vitamin C-deficient guinea pigs

L Hilsted; J F Rehfeld; T W Schwartz

doi:10.1016/0014-5793(86)80231-9

Impaired alpha-carboxyamidation of gastrin in vitamin C-deficient guinea pigs

L Hilsted, J F Rehfeld, T W Schwartz

Abstract

The biosynthetic activation of many hormonal peptides requires enzymatic alpha-carboxyamidation via glycine-extended intermediate forms. By specific radioimmunochemical measurement on extracts of guinea pig antra, we found that vitamin C deficiency induced a 30-fold increase in glycine-extended (i.e. inactive) gastrins. The results indicate that ascorbic acid is necessary for alpha-carboxyamidation in vivo, and thus, that the biosynthetic activation of hormonal peptides can be modified by simple dietary means.

Originalsprog	Engelsk
Tidsskrift	FEBS Letters
Vol/bind	196
Udgave nummer	1
Sider (fra-til)	151-4
Antal sider	4
ISSN	0014-5793
DOI	https://doi.org/10.1016/0014-5793(86)80231-9
Status	Udgivet - 3 feb. 1986
Udgivet eksternt	Ja

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10.1016/0014-5793(86)80231-9

Citationsformater

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title = "Impaired alpha-carboxyamidation of gastrin in vitamin C-deficient guinea pigs",

abstract = "The biosynthetic activation of many hormonal peptides requires enzymatic alpha-carboxyamidation via glycine-extended intermediate forms. By specific radioimmunochemical measurement on extracts of guinea pig antra, we found that vitamin C deficiency induced a 30-fold increase in glycine-extended (i.e. inactive) gastrins. The results indicate that ascorbic acid is necessary for alpha-carboxyamidation in vivo, and thus, that the biosynthetic activation of hormonal peptides can be modified by simple dietary means.",

keywords = "Animals, Ascorbic Acid/physiology, Diet, Gastrins/metabolism, Glycine/metabolism, Guinea Pigs, Peptide Fragments/analysis, Scurvy/metabolism",

author = "L Hilsted and Rehfeld, {J F} and Schwartz, {T W}",

year = "1986",

month = feb,

day = "3",

doi = "10.1016/0014-5793(86)80231-9",

language = "English",

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journal = "FEBS Letters",

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TY - JOUR

T1 - Impaired alpha-carboxyamidation of gastrin in vitamin C-deficient guinea pigs

AU - Hilsted, L

AU - Rehfeld, J F

AU - Schwartz, T W

PY - 1986/2/3

Y1 - 1986/2/3

N2 - The biosynthetic activation of many hormonal peptides requires enzymatic alpha-carboxyamidation via glycine-extended intermediate forms. By specific radioimmunochemical measurement on extracts of guinea pig antra, we found that vitamin C deficiency induced a 30-fold increase in glycine-extended (i.e. inactive) gastrins. The results indicate that ascorbic acid is necessary for alpha-carboxyamidation in vivo, and thus, that the biosynthetic activation of hormonal peptides can be modified by simple dietary means.

AB - The biosynthetic activation of many hormonal peptides requires enzymatic alpha-carboxyamidation via glycine-extended intermediate forms. By specific radioimmunochemical measurement on extracts of guinea pig antra, we found that vitamin C deficiency induced a 30-fold increase in glycine-extended (i.e. inactive) gastrins. The results indicate that ascorbic acid is necessary for alpha-carboxyamidation in vivo, and thus, that the biosynthetic activation of hormonal peptides can be modified by simple dietary means.

KW - Animals

KW - Ascorbic Acid/physiology

KW - Diet

KW - Gastrins/metabolism

KW - Glycine/metabolism

KW - Guinea Pigs

KW - Peptide Fragments/analysis

KW - Scurvy/metabolism

U2 - 10.1016/0014-5793(86)80231-9

DO - 10.1016/0014-5793(86)80231-9

M3 - Journal article

C2 - 3943627

SN - 0014-5793

VL - 196

SP - 151

EP - 154

JO - FEBS Letters

JF - FEBS Letters

IS - 1

ER -

Impaired alpha-carboxyamidation of gastrin in vitamin C-deficient guinea pigs

Abstract

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