Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

Impact of Metronidazole Treatment and Dientamoeba Fragilis Colonization on gut Microbiota Diversity

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Safety of High-Volume Plasmapheresis in Children With Acute Liver Failure

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Integration of eHealth Into Pediatric Inflammatory Bowel Disease Care is Safe: 3 Years of Follow-up of Daily Care

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Outcomes Following Pouch Formation in Paediatric Ulcerative Colitis: A Study from the Porto Group of ESPGHAN

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Extraintestinal Manifestations are Associated with Disease Severity in Pediatric Onset Inflammatory Bowel Disease

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  5. European Society Paediatric Gastroenterology, Hepatology and Nutrition Guidelines for Diagnosing Coeliac Disease 2020

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Stability and resilience of the intestinal microbiota in children in daycare - a 12 month cohort study

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Dientamoeba fragilis - a Commensal in Children in Danish Day Care Centers

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Childhood diarrhoea in Danish day care centres could be associated with infant colic, low birth weight and antibiotics

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Enteroaggregative Escherichia coli in Daycare-A 1-Year Dynamic Cohort Study

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  • Helle Gotfred-Rasmussen
  • Christen Rune Stensvold
  • Anna Cäcilia Ingham
  • Thor Bech Johannesen
  • Lee O'Brien Andersen
  • Dennis Röser
  • Henrik Vedel Nielsen
Vis graf over relationer

OBJECTIVES: The intestinal parasite Dientamoeba fragilis is a common colonizer of children in Denmark. Metronidazole has been used to reduce gastrointestinal symptoms in children colonized with D fragilis. We aimed to identify gut microbiota changes associated with D fragilis carrier status and metronidazole treatment of D fragilis-positive children.

METHODS: The fecal microbiota of 275 fecal samples from children treated with metronidazole (n = 48) or placebo (n = 48) were characterized by ribosomal DNA sequencing. Samples collected before (T1), 2 weeks after (T2), and 8 weeks (T5) after treatment were included. Seventy fecal samples from 70 age-matched parasite-negative children served as controls.

RESULTS: The abundance of 24 bacterial genera differed significantly according to D fragilis carrier status, with Flavonifractor being remarkably more abundant in children testing negative for D fragilis. Eight bacterial genera changed significantly in abundance in children losing versus keeping D fragilis after metronidazole treatment. Of these, 7 returned to pretreatment (T1) levels at T5. Meanwhile, the abundance of Flavonifractor continued to differ at T5, whereas for Ruminococcus the abundance only remained high in children who were D fragilis-negative at T2 and T5. Increases in Hungatella, Sutterella, and Streptococcus abundances observed at T2 were specific to metronidazole exposure and hence independent of D fragilis colonization.

CONCLUSIONS: This study revealed that specific bacterial genera were associated with D fragilis colonization. Metronidazole treatment had a short-term impact on the abundance of some bacterial genera, with most of these reverting to pretreatment levels 8 weeks after completed treatment.

OriginalsprogEngelsk
TidsskriftJournal of Pediatric Gastroenterology and Nutrition
Vol/bind73
Udgave nummer1
Sider (fra-til)23-29
Antal sider7
ISSN0277-2116
DOI
StatusUdgivet - 1 jul. 2021

ID: 64081230