Background and aim: The wireless motility capsule (WMC) is a non-invasive and radiation-free method for measuring gut transit in response to ingestion of a granola bar (SmartBar®). The WMC has the potential to measure gastrointestinal (GI) transit in metabolic research as part of a standardized mixed meal tolerance test. We aimed to evaluate GI transit with the WMC and postprandial plasma/serum concentrations of metabolites and GI hormones following ingestion of a SmartBar® versus a standardized mixed meal. Materials and methods: 14 healthy, normal weight participants completed a two-day crossover study. Following ingestion of either a SmartBar® or a standardized mixed meal, participants swallowed the WMC. Blood samples were drawn in the fasted state and at several time points postprandially for analyses of GI hormones and metabolites. Results: Median [Q1; Q3] GET was 98.0 [70.0; 113.0] min longer and iAUC of triglyceride, glucose-dependent insulinotropic polypeptide and peptide YY were greater after the standardized mixed meal compared to the SmartBar® (all P<0.001). Small bowel-, colonic- and whole gut transit times did not differ between meal types. Conclusion: The WMC can be used in combination with a standardized mixed meal for evaluation of gastrointestinal transit in healthy individuals. Gastric emptying time was prolonged in response to the standardized mixed meal while transit times of the small bowel, colon and whole gut did not differ between the test meals.