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Region Hovedstaden - en del af Københavns Universitetshospital
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Immunohistochemical localization of a gap junction protein (connexin43) in the muscularis externa of murine, canine, and human intestine

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  2. Analysis of enteroendocrine cell populations in the human colon

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  3. Circadian oscillators in the mouse brain: molecular clock components in the neocortex and cerebellar cortex

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  1. The association of celiac disease and allergic disease in a general adult population

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  2. A low-gluten diet induces changes in the intestinal microbiome of healthy Danish adults

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  3. The distribution of HLA DQ2 and DQ8 haplotypes and their association with health indicators in a general Danish population

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Electron-microscopic studies have revealed a heterogeneous distribution of gap junctions in the muscularis externa of mammalian intestines. This heterogeneity is observed at four different levels: among species; between small and large intestines; between longitudinal and circular muscle layers; and between subdivisions of the circular muscle layer. We correlated results obtained with two immunomethods, using an antibody to the known gap-junctional protein (connexin43) with ultrastructural findings, and further evaluated the respective sensitivity of these two approaches. For comparative reasons we also included the vascular smooth muscle of coronary arteries into our study. Two versions of the immunotechnique (peroxidase-antiperoxidase and fluorescence methods) were applied to frozen sections of murine, canine, and human small and large intestines, as well as to pig coronary artery. In the small intestine of all three species a very strong reactivity marked the outer main division of the circular muscle layer, while the longitudinal muscle layer as well as the inner thin division of the circular muscle layer were negative. In murine and human colon both muscle layers were negative, while in canine colon the border layer between the circular muscle and the submucosa reacted strongly, and scattered activity was found in the portion of the circular muscle layer (one tenth of its thickness) closest to the submucosa. The remainder of the circular muscle layer and the entire longitudinal muscle layer were negative in the canine colon. In the coronary artery we could not confirm the positive, specific labeling reported by other investigators (l.c.).(ABSTRACT TRUNCATED AT 250 WORDS)
OriginalsprogEngelsk
TidsskriftCell and Tissue Research
Vol/bind274
Udgave nummer2
Sider (fra-til)249-56
Antal sider8
ISSN0302-766X
StatusUdgivet - nov. 1993

ID: 39696471