Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

Imaging N-Acyl Homoserine Lactone Quorum Sensing In Vivo

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Determination of Binding Kinetics of Intrinsically Disordered Proteins by Surface Plasmon Resonance

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Analysis of Mass Cytometry Data

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Assessment of Peptidylarginine Deiminase Activity by ELISA Using Human Fibrinogen as Substrate

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Full-Length Open Reading Frame Amplification of Hepatitis C Virus

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  5. In Vitro Neutralization Assay Using Cultured Hepatitis C Virus

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. The efficacy of topical agents used in wounds for managing chronic biofilm infections: A systematic review

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. The environmental occurrence of Pseudomonas aeruginosa

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Biofilms of Mycobacterium abscessus complex can be sensitized to antibiotics by disaggregation and oxygenation

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Minimum information guideline for spectrofotometric and fluorometric methods to assess biofilm formation in microplates

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  5. Is pseudarthrosis after spinal instrumentation caused by a chronic infection?

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Vis graf over relationer

In order to study N-acyl homoserine lactone (AHL)-based quorum sensing in vivo, we present a protocol using an Escherichia coli strain equipped with a luxR-based monitor system, which in the presence of exogenous AHL molecules expresses a green fluorescent protein (GFP). Lungs from mice challenged intratracheally with alginate beads containing both a Pseudomonas aeruginosa strain together with the E. coli monitor strain can be investigated at different time points postinfection. Epifluorescent or confocal scanning laser microscopy (CSLM) is used to detect the GFP-expressing E. coli monitor strain in the lung tissues, indicating production and excretion of AHLs in vivo by the infecting P. aeruginosa.

OriginalsprogEngelsk
TidsskriftMethods in molecular biology
Vol/bind1673
Sider (fra-til)203-212
Antal sider10
ISSN1064-3745
DOI
StatusUdgivet - 2018

ID: 52090106