Imaging cerebral 2-ketoisocaproate metabolism with hyperpolarized (13)C magnetic resonance spectroscopic imaging

Sadia Asghar Butt; Lise V Søgaard; Peter O Magnusson; Mette H Lauritzen; Christoffer Laustsen; Per Åkeson; Jan Henrik Ardenkjaer-Larsen

doi:10.1038/jcbfm.2012.34

Imaging cerebral 2-ketoisocaproate metabolism with hyperpolarized (13)C magnetic resonance spectroscopic imaging

Sadia Asghar Butt, Lise V Søgaard, Peter O Magnusson, Mette H Lauritzen, Christoffer Laustsen, Per Åkeson, Jan Henrik Ardenkjaer-Larsen

MR-Videncenter AHH

32 Citationer (Scopus)

Abstract

The branched chain amino acid transaminase (BCAT) has an important role in nitrogen shuttling and glutamate metabolism in the brain. The purpose of this study was to describe the cerebral distribution and metabolism of hyperpolarized 2-keto[1-13C]isocaproate (KIC) in the normal rat using magnetic resonance modalities. Hyperpolarized KIC is metabolized to [1-13C]leucine (leucine) by BCAT. The results show that KIC and its metabolic product, leucine, are present at imageable quantities 20 seconds after end of KIC administration throughout the brain. Further, significantly higher metabolism was observed in hippocampal regions compared with the muscle tissue. In conclusion, the cerebral metabolism of hyperpolarized KIC is imaged and hyperpolarized KIC may be a promising substrate for evaluation of cerebral BCAT activity in conjunction with neurodegenerative disease.

Originalsprog	Engelsk
Tidsskrift	Journal of Cerebral Blood Flow and Metabolism
Vol/bind	32
Udgave nummer	8
Sider (fra-til)	1508-14
Antal sider	7
ISSN	0271-678X
DOI	https://doi.org/10.1038/jcbfm.2012.34
Status	Udgivet - 2012

Emneord

BCAT; 13C; hyperpolarization; magnetic resonance; metabolism

Adgang til dokumentet

10.1038/jcbfm.2012.34

Citationsformater

@article{ee93bd88fee543efb31aa7d85b2a3185,

title = "Imaging cerebral 2-ketoisocaproate metabolism with hyperpolarized (13)C magnetic resonance spectroscopic imaging",

abstract = "The branched chain amino acid transaminase (BCAT) has an important role in nitrogen shuttling and glutamate metabolism in the brain. The purpose of this study was to describe the cerebral distribution and metabolism of hyperpolarized 2-keto[1-(13)C]isocaproate (KIC) in the normal rat using magnetic resonance modalities. Hyperpolarized KIC is metabolized to [1-(13)C]leucine (leucine) by BCAT. The results show that KIC and its metabolic product, leucine, are present at imageable quantities 20 seconds after end of KIC administration throughout the brain. Further, significantly higher metabolism was observed in hippocampal regions compared with the muscle tissue. In conclusion, the cerebral metabolism of hyperpolarized KIC is imaged and hyperpolarized KIC may be a promising substrate for evaluation of cerebral BCAT activity in conjunction with neurodegenerative disease.",

keywords = "Animals, Biotransformation, Brain, Carbon Isotopes, Keto Acids, Leucine, Magnetic Resonance Imaging, Magnetic Resonance Spectroscopy, Molecular Structure, Rats, Rats, Wistar, Transaminases, BCAT; 13C; hyperpolarization; magnetic resonance; metabolism",

author = "{Asghar Butt}, Sadia and S{\o}gaard, {Lise V} and Magnusson, {Peter O} and Lauritzen, {Mette H} and Christoffer Laustsen and Per {\AA}keson and Ardenkjaer-Larsen, {Jan Henrik}",

year = "2012",

doi = "10.1038/jcbfm.2012.34",

language = "English",

volume = "32",

pages = "1508--14",

journal = "Journal of Cerebral Blood Flow and Metabolism",

issn = "0271-678X",

publisher = "SAGE Publications Inc.",

number = "8",

}

TY - JOUR

T1 - Imaging cerebral 2-ketoisocaproate metabolism with hyperpolarized (13)C magnetic resonance spectroscopic imaging

AU - Asghar Butt, Sadia

AU - Søgaard, Lise V

AU - Magnusson, Peter O

AU - Lauritzen, Mette H

AU - Laustsen, Christoffer

AU - Åkeson, Per

AU - Ardenkjaer-Larsen, Jan Henrik

PY - 2012

Y1 - 2012

N2 - The branched chain amino acid transaminase (BCAT) has an important role in nitrogen shuttling and glutamate metabolism in the brain. The purpose of this study was to describe the cerebral distribution and metabolism of hyperpolarized 2-keto[1-(13)C]isocaproate (KIC) in the normal rat using magnetic resonance modalities. Hyperpolarized KIC is metabolized to [1-(13)C]leucine (leucine) by BCAT. The results show that KIC and its metabolic product, leucine, are present at imageable quantities 20 seconds after end of KIC administration throughout the brain. Further, significantly higher metabolism was observed in hippocampal regions compared with the muscle tissue. In conclusion, the cerebral metabolism of hyperpolarized KIC is imaged and hyperpolarized KIC may be a promising substrate for evaluation of cerebral BCAT activity in conjunction with neurodegenerative disease.

AB - The branched chain amino acid transaminase (BCAT) has an important role in nitrogen shuttling and glutamate metabolism in the brain. The purpose of this study was to describe the cerebral distribution and metabolism of hyperpolarized 2-keto[1-(13)C]isocaproate (KIC) in the normal rat using magnetic resonance modalities. Hyperpolarized KIC is metabolized to [1-(13)C]leucine (leucine) by BCAT. The results show that KIC and its metabolic product, leucine, are present at imageable quantities 20 seconds after end of KIC administration throughout the brain. Further, significantly higher metabolism was observed in hippocampal regions compared with the muscle tissue. In conclusion, the cerebral metabolism of hyperpolarized KIC is imaged and hyperpolarized KIC may be a promising substrate for evaluation of cerebral BCAT activity in conjunction with neurodegenerative disease.

KW - Animals

KW - Biotransformation

KW - Brain

KW - Carbon Isotopes

KW - Keto Acids

KW - Leucine

KW - Magnetic Resonance Imaging

KW - Magnetic Resonance Spectroscopy

KW - Molecular Structure

KW - Rats

KW - Rats, Wistar

KW - Transaminases

KW - BCAT; 13C; hyperpolarization; magnetic resonance; metabolism

U2 - 10.1038/jcbfm.2012.34

DO - 10.1038/jcbfm.2012.34

M3 - Journal article

C2 - 22453630

SN - 0271-678X

VL - 32

SP - 1508

EP - 1514

JO - Journal of Cerebral Blood Flow and Metabolism

JF - Journal of Cerebral Blood Flow and Metabolism

IS - 8

ER -

Imaging cerebral 2-ketoisocaproate metabolism with hyperpolarized (13)C magnetic resonance spectroscopic imaging

Abstract

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