Identification of outer membrane Porin D as a vitronectin-binding factor in cystic fibrosis clinical isolates of Pseudomonas aeruginosa

Magnus Paulsson, Birendra Singh, Tamim Al-Jubair, Yu-Ching Su, Niels Høiby, Kristian Riesbeck

16 Citationer (Scopus)

Abstract

BACKGROUND: Pseudomonas aeruginosa is a pathogen that frequently colonizes patients with cystic fibrosis (CF) or chronic obstructive pulmonary disease (COPD). Several pathogens are known to bind vitronectin to increase their virulence. Vitronectin has been shown to enhance P. aeruginosa adhesion to host epithelial cells.

METHODS: We screened clinical isolates from the airways of CF patients and from the bloodstream of patients with bacteremia for binding of vitronectin. Two-dimensional SDS-PAGE and a proteomic approach were used to identify vitronectin-receptors in P. aeruginosa.

RESULTS: P. aeruginosa from the airways of CF patients (n=27) bound more vitronectin than bacteremic isolates (n=15, p=0.025). Porin D (OprD) was identified as a vitronectin-binding protein. A P. aeruginosa oprD transposon insertion mutant had a decreased binding to soluble and immobilized vitronectin (p≤0.001).

CONCLUSIONS: P. aeruginosa isolates obtained from CF patients significantly bound vitronectin. Porin D was defined as a novel P. aeruginosa vitronectin-receptor, and we postulate that the Porin D-dependent interaction with vitronectin may be important for colonization.

OriginalsprogEngelsk
TidsskriftJournal of cystic fibrosis : official journal of the European Cystic Fibrosis Society
Vol/bind14
Udgave nummer5
Sider (fra-til)600-7
Antal sider8
ISSN1569-1993
DOI
StatusUdgivet - sep. 2015

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