Identification of IFN-γ-producing CD4+ T cells following PMA stimulation

K. Kemp*, H. Bruunsgaard

*Corresponding author af dette arbejde
31 Citationer (Scopus)

Abstract

Treatment of T cells with phorbol esters, such as phorbol myristate acetate (PMA), induces downregulation of CD4, making unambiguous identification of this subset difficult. In this study, the kinetics of intracellular expression of interferon-γ (IFN-γ) and downmodulation of surface CD4 were measured in peripheral blood mononuclear cells (PBMC) after PMA stimulation. The number of IFN-γ-producing cells increased within a 4-h period while the fluorescence intensity of the CD4+ cell population decreased, and the two phenomena were correlated (n = 9; p = 0.01). Our data suggest that intracellular staining of CD4 together with cytokine staining will make identification of CD4+ cells possible and facilitate the procedure of intracellular staining of cytokines.

OriginalsprogEngelsk
TidsskriftJournal of Interferon and Cytokine Research
Vol/bind21
Udgave nummer7
Sider (fra-til)503-506
Antal sider4
ISSN1079-9907
DOI
StatusUdgivet - 2001
Udgivet eksterntJa

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